RegulonDB RegulonDB 10.8: Operon Form
   

uidABC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: uidABC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: uidABC
Gene(s): uidC, uidB, uidA   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Blanco C., et al., 1982
[2] Blattner FR., et al., 1997
Promoter
Name: uidAp
+1: 1696152
Distance from start of the gene: 81
Sequence: gttttttgatcgcggtgtcagttctttttatttccatttctcttccatgggtttctcacaGataactgtgtgcaacacaga
Evidence: [TIM]
Reference(s): [3] Blanco C. 1987
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator uidAp 1696231 1696253 -89.5 gttttaaagaTTAATGCGATCTATATCACGCTGtgggtattgc nd [AIBSCS], [APIORCISFBSCS], [CV(GEA/ROMA)], [GEA] [4], [6], [7]
[UidR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote UidR1 repressor uidAp 1696298 1696313 -153.5 tcgttcccaaTACGCTCGAACGAACGttcggttgct nd [GEA] [3], [4], [5]
[UxuR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal UxuR1 repressor uidAp 1696116 1696133 28.5 gtgcaacacaGAATTGGTTAACTAATCAgattaaaggt nd [APIORCISFBSCS], [CV(GEA/ROMA)], [GEA] [3], [4], [5], [6]
Note(s): 1Expression of the regulated gene is affected as a result of mutating the regulatory protein. Note that this evidence does not eliminate the possibility of an indirect effect of the regulator on the regulated gene.1Expression of the regulated gene is affected as a result of mutating the regulatory protein. Note that this evidence does not eliminate the possiblity of an indirect effect of the regulator on the regulated gene.1Expression of the regulated gene is affected as a result of mutating the regulatory protein. Note that this evidence does not eliminate the possibility of an indirect effect of the regulator on the regulated gene.
3Expression of the regulated gene is affected as a result of mutating the regulatory protein. Note that this evidence does not eliminate the possiblity of an indirect effect of the regulator on the regulated gene.


Evidence    

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [GEA] Gene expression analysis


Reference(s)    

 [1] Blanco C., Ritzenthaler P., Mata-Gilsinger M., 1982, Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene., J Bacteriol 149(2):587-94

 [2] Blattner FR., Plunkett G., Bloch CA., Perna NT., Burland V., Riley M., Collado-Vides J., Glasner JD., Rode CK., Mayhew GF., Gregor J., Davis NW., Kirkpatrick HA., Goeden MA., Rose DJ., Mau B., Shao Y., 1997, The complete genome sequence of Escherichia coli K-12., Science 277(5331):1453-74

 [3] Blanco C., 1987, Transcriptional and translational signals of the uidA gene in Escherichia coli K12., Mol Gen Genet 208(3):490-8

 [4] Blanco C., Mata-Gilsinger M., Ritzenthaler P., 1985, The use of gene fusions to study the expression of uidR, a negative regulatory gene of Escherichia coli K-12., Gene 36(1-2):159-67

 [5] Blanco C., Ritzenthaler P., Mata-Gilsinger M., 1986, Negative dominant mutations of the uidR gene in Escherichia coli: genetic proof for a cooperative regulation of uidA expression., Genetics 112(2):173-82

 [6] Rodionov DA., Mironov AA., Rakhmaninova AB., Gelfand MS., 2000, Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria., Mol Microbiol 38(4):673-83

 [7] Tsai MJ., Wang JR., Yang CD., Kao KC., Huang WL., Huang HY., Tseng CP., Huang HD., Ho SY., 2018, PredCRP: predicting and analysing the regulatory roles of CRP from its binding sites in Escherichia coli., Sci Rep 8(1):951

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