RegulonDB RegulonDB 10.9: Operon Form
   

mhpR-lacI operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mhpR-lacI
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: lacI
Gene(s): lacI   Genome Browser M3D Gene expression COLOMBOS
Note(s):
The lac operon and its network have been used to identify and characterize adaptive changes in lactose utilization in evolved populations 22253602
Promoter
Name: lacIp
+1: 367538
Distance from start of the gene: 28
Sequence: catttacgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccGgaagagagtcaattcagggt
Evidence: [TIM]
Reference(s): [1] Steege DA. 1977


Transcription unit       
Name: mhpR-lacI
Gene(s): lacI, mhpR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: mhpRp
+1: 368528
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 108
Sequence: aaaatgtcgttgcgcgcacagtacagcgcaacttattttgttaaaaacatgtaaatgattTtttattgtgcgctcagtata
                                 -35                  -10   +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[3] Torres B., et al., 2003


Transcription unit          
Name: mhpR-lacI
Gene(s): lacI, mhpR   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: mhpRp1
+1: 368536
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 116
Sequence: actcggacaaaatgtcgttgcgcgcacagtacagcgcaacttattttgttaaaaacatgtAaatgattttttattgtgcgc
                             -35                  -10       +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[4] Manso I., et al., 2011
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator mhpRp1 368566 368587 -40.5 cgactcggacAAAATGTCGTTGCGCGCACAGTAcagcgcaact nd [APIORCISFBSCS], [BPP], [GEA] [4]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
Evidence: [ICA] Inferred by computational analysis
Reference(s): [5] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -10.1 368547 368580 acaaaatgtcGTTGCGCGCACAGTACAGCGCAACTTATTTTGTtaaaaacatg
  anti-terminator -4.7 368571 368616 ggtgttgtatTGCCATTTCAGTACCTCACGACTCGGACAAAATGTCGTTGCGCGCacagtacagc
  anti-anti-terminator -11.0 368613 368650 tgaacgctatGGTTAACAGCAGGCTGGATGTCAGGGTGTTGTATTGCcatttcagta
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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