RegulonDB RegulonDB 10.8: Operon Form
   

argO operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: argO
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: argO
Gene(s): argO   Genome Browser M3D Gene expression COLOMBOS
Note(s): The activation by both Lrp and ArgP in the argO operator is intertwined, each activator being more potent in the absence of the other Peeters E,2009 The competitive activation of a single promoter by two activator proteins, as described by Peeters et al., appears to be rare Peeters E,2009 The three sites of the Lrp activator act cooperatively and normally in wrapping of the operator DNA in the argO promoter region Peeters E,2009 Expression of argO is modulated by the effectors of both activators, which ensures an adapted and fine-tuned synthesis of the transporter in response to environmental conditions Peeters E,2009
In 1999 Laishram et al. proposed that ArgP binds to a region of approximately 60 bp in the intergenic region of argO Laishram RS,2007. Peeters E,2009. The curator assigned two binding sites of 20 bp for ArgP, located at -33.5 and -53.5, and proposes that in this intergenic region this regulator binds as dimer of dimers to two contiguous degenerated inverted repeat sites. The curator identified the consensus sequence of ArgP and assigned the possible central position of these binding sites, based on similarity to consensus sequences.
ArgP has been identified as binding as a dimer of dimers in the intergenic region of dnaA in E. coli Lee Y,1997 and also in the regulatory region of gdhA of Klebsiella pneumoniae 18424527. For this reason, there is a possibility that this regulator transcriptionally regulates as a dimer of dimers.
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: argOp
+1: 3068836
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 28
Sequence: ctgattgccaattaatattatcaatttccgctaataacaatcccgcgatatagtctctgcAtcagatacttaattcggaat
                         -35                     -10        +1                   
Note(s): In the argO and dapB promoters, the lysine repression of ArgP-dependent transcription between argO and dapB is apparently different. In the dapBp1 promoter, lysine prevents binding of ArgP Bouvier J,2008 whereas in the argO promoter, lysine traps RNA polymerase recruitment by ArgP Laishram RS,2007.
Evidence: [CV(RPF/TA)]
[CV(RPF/TIM)]
[CV(TA/TIM)]
[HIPP]
[HTIM]
[RPF]
[TIM]
Reference(s): [1] Laishram RS., et al., 2007
[2] Mendoza-Vargas A., et al., 2009
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArgP-arginine1 activator argOp 3068860 3068879 -33.5 ccaattaataTTATCAATTTCCGCTAATAAcaatcccgcg nd [APIORCISFBSCS], [BCE], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(CHIP-SV/SM)], [CV(GEA/ROMA)], [CV(GEA/ROMA/SM)], [GEA], [SM] [1], [3], [4], [5], [6], [7]
proximal ArgP-arginine activator argOp 3068880 3068900 -53.5 tcttattagtTTTTCTGATTGCCAATTAATAttatcaattt nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(CHIP-SV/SM)], [CV(GEA/ROMA)], [CV(GEA/ROMA/SM)], [GEA], [IC], [SM] [1], [3], [4], [5], [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Lrp-leucine activator argOp 3068873 3068889 -44.5 tttctgattgCCAATTAATATTATCAAtttccgctaa nd [APIORCISFBSCS], [BPP] [3]
proximal Lrp-leucine activator argOp 3068894 3068910 -65.5 ctggcctctcTCTTATTAGTTTTTCTGattgccaatt nd [APIORCISFBSCS], [BPP] [3]
proximal Lrp-leucine activator argOp 3068918 3068934 -89.5 aacgctgaggCCAGATAATACTCTCTGgcctctctct nd [BPP], [GEA] [3]
Note(s): 1The transcription of argO is induced by ArgP when it is bound to arginine, but it is inhibited when lysine is bound to ArgP Laishram RS,2007.
ArgP regulates the argO operon by a mechanism involving active trapping at the argOp promoter by ArgP of RNA polymerase, which is then prevented from being released and engaging in productive transcription Laishram RS,2007. Marbaniang CN,2011

5The transcription of argO is induced by ArgP when it is bound to arginine, but it is inhibited when lysine is bound to ArgP Laishram RS,2007.
ArgP regulates the argO operon by a mechanism involving active trapping at the argOp promoter by ArgP of RNA polymerase, which is then prevented from being released and engaging in productive transcription Laishram RS,2007. Marbaniang CN,2011




Reference(s)    

 [1] Laishram RS., Gowrishankar J., 2007, Environmental regulation operating at the promoter clearance step of bacterial transcription., Genes Dev 21(10):1258-72

 [2] Mendoza-Vargas A., Olvera L., Olvera M., Grande R., Vega-Alvarado L., Taboada B., Jimenez-Jacinto V., Salgado H., Juarez K., Contreras-Moreira B., Huerta AM., Collado-Vides J., Morett E., 2009, Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli., PLoS One 4(10):e7526

 [3] Peeters E., Nguyen Le Minh P., Foulquie-Moreno M., Charlier D., 2009, Competitive activation of the Escherichia coli argO gene coding for an arginine exporter by the transcriptional regulators Lrp and ArgP., Mol Microbiol 74(6):1513-26

 [4] Marbaniang CN., Gowrishankar J., 2011, Role of ArgP (IciA) in Lysine-Mediated Repression in Escherichia coli., J Bacteriol 193(21):5985-96

 [5] Nandineni MR., Gowrishankar J., 2004, Evidence for an arginine exporter encoded by yggA (argO) that is regulated by the LysR-type transcriptional regulator ArgP in Escherichia coli., J Bacteriol 186(11):3539-46

 [6] Nguyen Le Minh P., Velazquez Ruiz C., Vandermeeren S., Abwoyo P., Bervoets I., Charlier D., 2018, Differential protein-DNA contacts for activation and repression by ArgP, a LysR-type (LTTR) transcriptional regulator in Escherichia coli., Microbiol Res 206:141-158

 [7] Bouvier J., Stragier P., Morales V., Remy E., Gutierrez C., 2008, Lysine represses transcription of the Escherichia coli dapB gene by preventing its activation by the ArgP activator., J Bacteriol 190(15):5224-9


RegulonDB