RegulonDB RegulonDB 11.1: Operon Form
   

mlrA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mlrA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mlrA
Gene(s): mlrA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: mlrAp
+1: 2214840
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 26
Sequence: tagatgcacaaaaaacgggcaaaactacctggttcgcaaaactgcgtctaaagttaaaccGggacctcgcgagcaagggtg
                         -35                     -10        +1                   
Note(s): Regulation of the mlrAp promoter has been described Brown PK,2001. The mlrA gene is RpoS regulated in an avian pathogenic Escherichia coli strain Brown PK,2001.
Evidence: [COMP-AINF]
[COMP-HINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[EXP-IEP]
Reference(s): [1] Brown PK., et al., 2001
[2] Huerta AM., et al., 2003
[3] Pesavento C., et al., 2012
TF binding sites (TFBSs)
[FliZ,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FliZ repressor mlrAp 2214816 2214845 -10.0 acctggttcgCAAAACTGCGTCTAAAGTTAAACCGGGACCtcgcgagcaa nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [3], [4]


Transcription unit          
Name: mlrA
Gene(s): mlrA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: mlrAp2
+1: 2214840
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 26
Sequence: tagatgcacaaaaaacgggcaaaactacctggttcgcaaaactgcgtctaaagttaaaccGggacctcgcgagcaagggtg
                          -35               -10             +1                   
Note(s): Regulation of the mlrAp promoter has been described Brown PK,2001. The mlrA gene is RpoS regulated in an avian pathogenic Escherichia coli strain Brown PK,2001.
Evidence: [COMP-AINF]
[COMP-HINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[EXP-IEP]
Reference(s): [1] Brown PK., et al., 2001
[2] Huerta AM., et al., 2003
[3] Pesavento C., et al., 2012
TF binding sites (TFBSs)
[FliZ,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FliZ repressor mlrAp2 2214816 2214845 -10.0 acctggttcgCAAAACTGCGTCTAAAGTTAAACCGGGACCtcgcgagcaa nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [3], [4]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF] Inferred by a human based on computational evidence

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IEP] Inferred from expression pattern

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Brown PK., Dozois CM., Nickerson CA., Zuppardo A., Terlonge J., Curtiss R., 2001, MlrA, a novel regulator of curli (AgF) and extracellular matrix synthesis by Escherichia coli and Salmonella enterica serovar Typhimurium., Mol Microbiol 41(2):349-63

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Pesavento C., Hengge R., 2012, The global repressor FliZ antagonizes gene expression by σS-containing RNA polymerase due to overlapping DNA binding specificity., Nucleic Acids Res 40(11):4783-93

 [4] Pesavento C., Becker G., Sommerfeldt N., Possling A., Tschowri N., Mehlis A., Hengge R., 2008, Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli., Genes Dev 22(17):2434-46

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