RegulonDB RegulonDB 10.9: Operon Form
   

abgABT-ogt operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: abgABT-ogt
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: ogt
Gene(s): ogt   Genome Browser M3D Gene expression COLOMBOS
Note(s): Derrick et al. (2009) suggested that induction of ogt by NarL alone in response to external nitrate provides a prophylactic effect against possible genotoxicity arising from nitrate metabolism Squire DJ,2009 NarL activates the ogt promoter independently of FNR Squire DJ,2009 NarL (E273 residue) appears to interact directly with RNA polymerase (α-CTD) to activate transcription of the ogt promoter Ruanto P,2020.
The ogt promoter is induced by NarL in response to nitrate ions, without help from FNR Squire DJ,2009
Based on experiments utilizing a mutant background, it was shown that NarP plays little or no role in the ogt promoter region Squire DJ,2009
Promoter
Name: ogtp
+1: 1400284
Distance from start of the gene: 48
Sequence:
Evidence: [IMP]
[TIM]
Reference(s): [1] Potter PM., et al., 1987
[2] Ruanto P., et al., 2020
TF binding sites (TFBSs)
[Fis,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis repressor ogtp 1400359 1400373 -82.0 gacgcgaaacTGGGTACTTACTATTCgttagtcttg nd [BPP] [3]
[NarL,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NarL-phosphorylated activator ogtp 1400322 1400329 -41.0 cttatcttttTGGTGGTAtggctgctga nd [BPP], [GEA], [SM] [2], [3]
proximal NarL-phosphorylated activator ogtp 1400331 1400338 -50.0 ccctatccacTTATCTTTttggtggtat nd [BPP], [GEA], [SM] [2], [3]
proximal NarL-phosphorylated activator ogtp 1400355 1400362 -74.0 ggtacttactATTCGTTAgtcttgccct nd [BPP], [GEA], [SM] [2], [3]
proximal NarL-phosphorylated activator ogtp 1400364 1400371 -83.0 gcgaaactggGTACTTACtattcgttag nd [BPP], [GEA], [SM] [2], [3]


Transcription unit       
Name: abgABT-ogt
Gene(s): ogt, abgT, abgB, abgA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Although a putative σ54-binding site was identified upstream of the abgB gene and the gene expression was affected in microarray experiments in a mutant
σ54 strain and a σ54-overexpressing strain, ChIP assays demonstrated that σ54 does not bind upstream of abgB Zhao K,2010 Therefore, we did not assign a σ54 promoter to this gene.
Evidence: [ICWHO] Inferred computationally without human oversight


Evidence    

 [IMP] Inferred from mutant phenotype

 [TIM] Transcription initiation mapping

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Potter PM., Wilkinson MC., Fitton J., Carr FJ., Brennand J., Cooper DP., Margison GP., 1987, Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli., Nucleic Acids Res 15(22):9177-93

 [2] Ruanto P., Chismon DL., Hothersall J., Godfrey RE., Lee DJ., Busby SJW., Browning DF., 2020, Activation by NarL at the Escherichia coli ogt promoter., Biochem J 477(15):2807-2820

 [3] Squire DJ., Xu M., Cole JA., Busby SJ., Browning DF., 2009, Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters., Biochem J 420(2):249-57

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