RegulonDB RegulonDB 11.1: Operon Form

lipA operon and associated TUs in Escherichia coli K-12 genome

Name: lipA
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit       
Name: lipA
Gene(s): lipA   Genome Browser M3D Gene expression COLOMBOS
Note(s): PdhR was identified by Kaleta et al. (2010) as a negative regulator of lipA, based on consensus sequences, microarrays, and electromobility shift analyses (EMSA) at bp 69 (designated by Feng and Cronan [2014] as lipA site 1) of the lipAp promoter Kaleta C,2010 However, Feng and Cronan (2014) reported that this site is nonfunctional and physiologically irrelevant. The binding detected by Kaleta et al. (2010) is due to another site (lipA site 3) that is located far upstream of the lipAp promoter and which is a half-palindrome with weak PdhR-binding ability Feng Y, Cronan JE,2014
Other possible PdhR-binding degenerate palindromes were identified by Feng and Cronan (2014). One of them is downstream of the lipAp promoter at positions 22 to 38 (lipA site 2) and the other one is at positions -293 to -277 (lipA site 3). All three of these sites were present in the EMSA probe used by Kaleta et al. (2010) and thus could contribute to the weak binding observed Feng Y, Cronan JE,2014 On the other hand, deletion of pdhR did not cause any detectable change in the lipA transcription level for β-galactosidase activity, indicating that PdhR plays no role in regulation of lipA expression Feng Y, Cronan JE,2014
It was concluded that lipA is not controlled by PdhR, based on in vivo and in vitro evidence Feng Y, Cronan JE,2014
Evidence: [COMP-AINF] Inferred computationally without human oversight
Name: lipAp
+1: 660301
Distance from start of the gene: 85
Sequence: ttacattttcgcgaccgctttggctgctttcaggtccgtttcaatgatatactgccagtcGttaattcaaaaatagttgat
Reference(s): [1] Mendoza-Vargas A., et al., 2009