RegulonDB RegulonDB 11.1: Operon Form
   

frr operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: frr
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: frr
Gene(s): frr   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [1] Shimizu I., et al., 1991
Promoter
Name: frrp
+1: 192814
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 58
Sequence: aatagcctaataatccagacgattacccgtaatatgtttaatcagggctatacttagcacActtccactgtgtgtgactgt
                       -35                       -10        +1                   
Evidence: [COMP-HINF]
[EXP-IDA]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[RS-EPT-CBR]
Reference(s): [2] Salgado H, et al., 2012
[1] Shimizu I., et al., 1991
Terminator(s)
Type: rho-independent
Sequence: tcttgaacgaCAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTctgtctcaac
Reference(s): [1] Shimizu I., et al., 1991
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition frrp nd   [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [3]
Evidence: [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
Reference(s): [3] Traxler MF., et al., 2008


Evidence    

 [COMP-HINF] Inferred by a human based on computational evidence

 [EXP-IDA] Inferred from direct assay

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Shimizu I., Kaji A., 1991, Identification of the promoter region of the ribosome-releasing factor cistron (frr)., J Bacteriol 173(16):5181-7

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Traxler MF., Summers SM., Nguyen HT., Zacharia VM., Hightower GA., Smith JT., Conway T., 2008, The global, ppGpp-mediated stringent response to amino acid starvation in Escherichia coli., Mol Microbiol 68(5):1128-48

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