RegulonDB RegulonDB 10.8: Operon Form
   

dsbG operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dsbG
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Gene(s): dsbG   Genome Browser M3D Gene expression COLOMBOS
Note(s): Expression of dsbG is activated by OxyR. This is also true for the expression of the divergently transcribed ahpC. The intergenic region between dsbG and ahpC carries two binding sites for OxyR, a dsbG-proximal site, located 54 bp upstream of the dsbG start codon, and an ahpC-proximal site, located 290 bp upstream of the dsbG start codon Zheng M,2001
Northern blotting and primer extension assays showed that OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts, while OxyR binding to the dsbG-proximal site leads to the induction of a second ahpC transcript. This transcript of ahpC and the transcript of dsbG overlap by over 100 nucleotides Zheng M,2001
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: dsbGp
+1: 638800
Distance from start of the gene: 227
Sequence: aagttttaccttacaaccttcgtaagacaactttgtgaactttgcaccgccagcggcgatAaaaagtaaaatggccccctt
Evidence: [TIM]
Reference(s): [1] Zheng M., et al., 2001
TF binding sites (TFBSs)
[OxyR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OxyR activator dsbGp 638847 638863 -55.0 ttatcaaatcGATAAGTTTTACCTTACaaccttcgta nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [1], [3], [4], [5]
proximal OxyR activator dsbGp 638869 638885 -77.0 cgattcggctAATGCGTTTCCATTATCaaatcgataa nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [1], [2], [3], [4], [5]


Evidence    

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(CHIP-SV/GEA/ROMA)] cross validation(CHIP-SV/GEA/ROMA)

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [GEA] Gene expression analysis


Reference(s)    

 [1] Zheng M., Wang X., Doan B., Lewis KA., Schneider TD., Storz G., 2001, Computation-directed identification of OxyR DNA binding sites in Escherichia coli., J Bacteriol 183(15):4571-9

 [2] Storz G., Tartaglia LA., Ames BN., 1990, Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation., Science 248(4952):189-94

 [3] Tartaglia LA., Gimeno CJ., Storz G., Ames BN., 1992, Multidegenerate DNA recognition by the OxyR transcriptional regulator., J Biol Chem 267(3):2038-45

 [4] Tartaglia LA., Storz G., Ames BN., 1989, Identification and molecular analysis of oxyR-regulated promoters important for the bacterial adaptation to oxidative stress., J Mol Biol 210(4):709-19

 [5] Toledano MB., Kullik I., Trinh F., Baird PT., Schneider TD., Storz G., 1994, Redox-dependent shift of OxyR-DNA contacts along an extended DNA-binding site: a mechanism for differential promoter selection., Cell 78(5):897-909

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