RegulonDB RegulonDB 10.8: Operon Form

thrU-tyrU-glyT-thrT-tufB operon and associated TUs in Escherichia coli K-12 genome

Name: thrU-tyrU-glyT-thrT-tufB
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: thrU-tyrU-glyT-thrT-tufB
Synonym(s): thrU
Gene(s): thrU, tyrU, glyT, thrT, tufB   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[ITC] Inferred through co-regulation
Reference(s): [1] Lee JS., et al., 1981
[2] van Delft JH., et al., 1987
Name: thrUp
+1: 4175381
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 7
Sequence: aaaacaaattatgcaattttttagttgcatgaactcgcatgtctccatagaatgcgcgctActtgatgccgacttagctca
                         -35                    -10         +1                   
Evidence: [HIPP]
Reference(s): [1] Lee JS., et al., 1981
[3] Mizushima-Sugano J., et al., 1985
Type: rho-independent
Sequence: caatgcgcacTAAAAGGGCATCATTTGATGCCCTTTTTgcacgctttc
Reference(s): [4] Fournier MJ., et al., 1985
[5] Hudson L., et al., 1981
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Fis activator thrUp 4175251 4175265 -123.0 gcacaatgatGTTGAAAAAGTGTGCtaatctgccc nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [2], [6], [7], [8]
proximal Fis activator thrUp 4175283 4175297 -91.0 ccctccgttcGGCTGTTTCTTCATCgtgtcgcata nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [2], [6], [7], [8]
proximal Fis activator thrUp 4175303 4175317 -71.0 tcatcgtgtcGCATAAAATGTGACCaataaaacaa nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA], [IHBCE] [2], [6], [7], [8], [9]
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition thrUp The nucleotide sequence (GCGC) from positions -7 to -4 (with respect to the transcription start site of the thrUp promoter) is responsible for the inhibition of the thrU-tyrU-glyT-thrT-tufB operon by ppGpp (0.2 μM) during stringent control Mizushima-Sugano J,1985 [IEP]
Evidence: [IEP] Inferred from expression pattern
[IMP] Inferred from mutant phenotype
Reference(s): [3] Mizushima-Sugano J., et al., 1985


 [1] Lee JS., An G., Friesen JD., Fill NP., 1981, Location of the tufB promoter of E. coli: cotranscription of tufB with four transfer RNA genes., Cell 25(1):251-8

 [2] van Delft JH., Marinon B., Schmidt DS., Bosch L., 1987, Transcription of the tRNA-tufB operon of Escherichia coli: activation, termination and antitermination., Nucleic Acids Res 15(22):9515-30

 [3] Mizushima-Sugano J., Kaziro Y., 1985, Regulation of the expression of the tufB operon: DNA sequences directly involved in the stringent control., EMBO J 4(4):1053-8

 [4] Fournier MJ., Ozeki H., 1985, Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12., Microbiol Rev 49(4):379-97

 [5] Hudson L., Rossi J., Landy A., 1981, Dual function transcripts specifying tRNA and mRNA., Nature 294(5840):422-7

 [6] Hengen PN., Bartram SL., Stewart LE., Schneider TD., 1997, Information analysis of Fis binding sites., Nucleic Acids Res 25(24):4994-5002

 [7] Nilsson L., Vanet A., Vijgenboom E., Bosch L., 1990, The role of FIS in trans activation of stable RNA operons of E. coli., EMBO J 9(3):727-34

 [8] Verbeek H., Nilsson L., Baliko G., Bosch L., 1990, Potential binding sites of the trans-activator FIS are present upstream of all rRNA operons and of many but not all tRNA operons., Biochim Biophys Acta 1050(1-3):302-6

 [9] Bosch L., Nilsson L., Vijgenboom E., Verbeek H., 1990, FIS-dependent trans-activation of tRNA and rRNA operons of Escherichia coli., Biochim Biophys Acta 1050(1-3):293-301