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Name: | mazEFG | |||||||||
Synonym(s): | chpRA | |||||||||
Gene(s): | mazG, mazF, mazE Genome Browser M3D Gene expression COLOMBOS | |||||||||
Note(s): | Based on deletion of rpoS, it has been shown that the stationary-phase sigma factor σs is responsible for resistance to chpRA-mazG-mediated cell death during the stationary growth phase Kolodkin-Gal I, Engelberg-Kulka H,2009 | |||||||||
Evidence: | [ITCR] Inferred through co-regulation [LTED] Length of transcript experimentally determined [PAGTSBP] Products of adjacent genes in the same biological process |
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Reference(s): |
[1] Aizenman E., et al., 1996 [2] Gross M., et al., 2006 [3] Marianovsky I., et al., 2001 [4] Masuda Y., et al., 1993 [5] Metzger S., et al., 1988 |
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Promoter | ||||||||||
Name: | mazEp2 | |||||||||
+1: | 2911355 | |||||||||
Distance from start of the gene: | 16 | |||||||||
Sequence: |
ccgaaatttgctcgtatctacaatgtagattgatatatactgtatctacatatgatagcgGtttgaggaaagggttatgat |
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Evidence: | [TIM] | |||||||||
Reference(s): |
[1] Aizenman E., et al., 1996 [3] Marianovsky I., et al., 2001 |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | MazE | repressor | mazEp2 | 2911363 | 2911388 | -20.0 | ctacaatgtaGATTGATATATACTGTATCTACATATgatagcggtt | nd | [GEA], [BCE], [SM] | [3] |
proximal | MazE | repressor | mazEp2 | 2911376 | 2911403 | -34.0 | gaaatttgctCGTATCTACAATGTAGATTGATATATACtgtatctaca | nd | [GEA], [BCE], [SM] | [3] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | MazE-MazF | repressor | mazEp2 | 2911362 | 2911389 | -20.0 | tctacaatgtAGATTGATATATACTGTATCTACATATGatagcggttt | nd | [GEA], [BCE], [SM] | [3] |
proximal | MazE-MazF | repressor | mazEp2 | 2911376 | 2911403 | -34.0 | gaaatttgctCGTATCTACAATGTAGATTGATATATACtgtatctaca | nd | [GEA], [BCE], [SM] | [3] |
Name: | mazEFG | |||||||||
Synonym(s): | chpRA | |||||||||
Gene(s): | mazG, mazF, mazE Genome Browser M3D Gene expression COLOMBOS | |||||||||
Note(s): | Based on deletion of rpoS, it has been shown that the stationary-phase sigma factor σs is responsible for resistance to chpRA-mazG-mediated cell death during the stationary growth phase Kolodkin-Gal I, Engelberg-Kulka H,2009 | |||||||||
Evidence: | [ITCR] Inferred through co-regulation [LTED] Length of transcript experimentally determined [PAGTSBP] Products of adjacent genes in the same biological process |
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Reference(s): |
[1] Aizenman E., et al., 1996 [2] Gross M., et al., 2006 [3] Marianovsky I., et al., 2001 [4] Masuda Y., et al., 1993 [5] Metzger S., et al., 1988 |
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Promoter | ||||||||||
Name: | mazEp1 | |||||||||
+1: | 2911368 | |||||||||
Distance from start of the gene: | 29 | |||||||||
Sequence: |
cacgggagttaggccgaaatttgctcgtatctacaatgtagattgatatatactgtatctAcatatgatagcggtttgagg |
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Evidence: |
[RS-EPT-CBR] [TIM] |
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Reference(s): |
[1] Aizenman E., et al., 1996 [3] Marianovsky I., et al., 2001 [6] Salgado H, et al., 2012 |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | MazE | repressor | mazEp1 | 2911363 | 2911388 | -7.0 | ctacaatgtaGATTGATATATACTGTATCTACATATgatagcggtt | nd | [BCE], [GEA], [SM], [BCE], [SM] | [3] |
proximal | MazE | repressor | mazEp1 | 2911376 | 2911403 | -21.0 | gaaatttgctCGTATCTACAATGTAGATTGATATATACtgtatctaca | nd | [BCE], [GEA], [SM], [BCE], [SM] | [3] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | MazE-MazF | repressor | mazEp1 | 2911362 | 2911389 | -7.0 | tctacaatgtAGATTGATATATACTGTATCTACATATGatagcggttt | nd | [BCE], [GEA], [SM], [BCE], [SM] | [3] |
proximal | MazE-MazF | repressor | mazEp1 | 2911376 | 2911403 | -21.0 | gaaatttgctCGTATCTACAATGTAGATTGATATATACtgtatctaca | nd | [BCE], [GEA], [SM], [BCE], [SM] | [3] |
Evidence: | [GEA] Gene expression analysis |
Reference(s): |
[1] Aizenman E., et al., 1996 [2] Gross M., et al., 2006 |
Name: | relA |
Gene(s): | relA Genome Browser M3D Gene expression COLOMBOS |
Evidence: | [IEP] Inferred from expression pattern |
Reference(s): | [7] Brown DR., et al., 2014 |
Promoter | |
Name: | relAp3 |
+1: | 2913700 |
Sigma Factor: | Sigma54 Sigmulon |
Distance from start of the gene: | 49 |
Sequence: |
tggatatgttcccacacacgggacatctggaatcgatggtacttttctcgcgcgttaaatAgttgcgatttgccgatttcg |
Evidence: |
[IDA] [IHBCE] [TIM] |
Reference(s): | [7] Brown DR., et al., 2014 |
Name: | relA-mazEF |
Synonym(s): | chpA, relA-chpRA |
Gene(s): | mazF, mazE, relA Genome Browser M3D Gene expression COLOMBOS |
Note(s): | Based on microarray analysis, Traxler et al. (2008) determined that relA positively regulates its own expression Traxler MF,2008 The control exercised by relA, on glycogen levels may result from an interplay of its (p)ppGpp-mediated positive effect on glgCAP transcription, deviation of surplus ATP flux from nucleic acid and protein biosynthetic pathways to glycogen production, and its negative effect on intracellular levels of the GlgC negative effector AMP Montero M, Eydallin G, Viale AM, Almagro G, Muñoz FJ, Rahimpour M, Sesma MT, Baroja-Fernández E, Pozueta-Romero J,2009 |
Evidence: | [IEP] Inferred from expression pattern [ITCR] Inferred through co-regulation |
Reference(s): |
[4] Masuda Y., et al., 1993 [5] Metzger S., et al., 1988 |
Promoter | |
Name: | relAp1 |
+1: | 2913829 |
Sigma Factor: | Sigma70 Sigmulon |
Distance from start of the gene: | 178 |
Sequence: |
gtgttatgcagcaaattataaaactggaacctattcgtatagtttatgtatcctgtaaccCtgcaacgctggctcgggata -35 -10 +1 |
Note(s): | The RelA production is controlled by two promoters, relAp1 and relAp2. relAp1 is a constitutive promoter, which is mainly responsible for RelA production during log phase. relAp2, which is fine-tuned by the global regulators CRP, H-NS, and RpoS, is induced temporarily at the transition state between the exponential growth and stationary phases. Both promoters are required for maximal accumulation of RelA during stationary phase. There is a UP element-like sequence immediately after the -35 box of the relAp1 promoter which is essential for promoter activity (based on a mutation of the putative UP element) Nakagawa A,2006. |
Evidence: |
[HTTIM] [ICWHO] [IHBCE] [TIM] |
Reference(s): |
[8] Huerta AM., et al., 2003 [9] Maciag A., et al., 2011 [5] Metzger S., et al., 1988 [10] Nakagawa A., et al., 2006 |
Name: | relA-mazEF | |||||||||
Gene(s): | mazF, mazE, relA Genome Browser M3D Gene expression COLOMBOS | |||||||||
Evidence: | [IEP] Inferred from expression pattern [ITCR] Inferred through co-regulation |
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Reference(s): |
[4] Masuda Y., et al., 1993 [5] Metzger S., et al., 1988 |
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Promoter | ||||||||||
Name: | relAp2 | |||||||||
+1: | 2914277 | |||||||||
Sigma Factor: | Sigma70 Sigmulon | |||||||||
Distance from start of the gene: | 626 | |||||||||
Sequence: |
ttttcgcattctgaaggcctggatctgtatctcgcccccgatagtgagatactcgaaaccGtctctggtgagatgccctgg -35 -10 +1 |
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Note(s): | Production of RelA is controlled by two promoters, relAp1 and relAp2. relAp1 is a constitutive promoter, which is mainly responsible for RelA production during log phase. relAp2, which is fine-tuned by the global regulators CRP, H-NS, and RpoS, is induced temporarily at the transition state between the exponential growth and stationary phases. Both promoters are required for maximal accumulation of RelA during stationary phase. Although relAp2 expression is partially repressed by RpoS after mid-log phase, this negative effect could be due to the RNA polymerase competition Nakagawa A,2006. However, this could also be an indirect effect. | |||||||||
Evidence: |
[CV(SM/TIM)] [HTTIM] [ICWHO] [IHBCE] [SM] [TIM] |
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Reference(s): |
[8] Huerta AM., et al., 2003 [9] Maciag A., et al., 2011 [10] Nakagawa A., et al., 2006 |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | CRP-cyclic-AMP | activator | relAp2 | 2914327 | 2914348 | -60.5 | gatcgcgaaaAACTGGAACGCTTTTCGCATTCtgaaggcctg | nd | [APIORCISFBSCS], [BPP], [SM] | [10] |
Evidence: | [IMP] Inferred from mutant phenotype |
Reference(s): | [12] Goodman C. 2012 |
Name: | relA |
Gene(s): | relA Genome Browser M3D Gene expression COLOMBOS |
Evidence: | [IEP] Inferred from expression pattern |
Reference(s): | [7] Brown DR., et al., 2014 |
Promoter | |
Name: | relAp4 |
+1: | 2914318 |
Sigma Factor: | Sigma54 Sigmulon |
Distance from start of the gene: | 667 |
Sequence: |
ccgcaccgctaagttcggcagatcgcgaaaaactggaacgcttttcgcattctgaaggccTggatctgtatctcgcccccg |
Evidence: |
[IDA] [TIM] |
Reference(s): | [7] Brown DR., et al., 2014 |
RNA cis-regulatory element | ![]() |
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Regulation, transcriptional elongation | |
Attenuator type: | Translational |
Strand: | reverse |
Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
---|---|---|---|---|---|
terminator | -9.4 | 2910707 | 2910732 | cttctattctGGTCACAGGTTTCCACCTGACAGACccgcaatttt |
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |