RegulonDB RegulonDB 10.8: Operon Form
   

clpS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: clpS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: clpS
Gene(s): clpS   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IHBCE] Inferred by a human based on computational evidence
Promoter
Name: clpSp
+1: 922751
Distance from start of the gene: 162
Sequence: gagaattagggcgagccgtcaagcatttgacaggggacaaggggcaggtatgaatcaaaaAtttgaagcagttaacgctat
Note(s): The transcription start site for clpSp was associated with a base pair on the TSS identified on Salmonella enterica serovar Typhimurium 27564394.
Evidence: [IC]
Reference(s): [1] Yeom J., et al., 2018
TF binding sites (TFBSs)
[PhoP,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoP-Phosphorylated1 repressor clpSp 922736 922752 -7.0 gacaaggggcAGGTATGAATCAAAAATttgaagcagt nd [BCE], [CV(GEA/ROMA)], [GEA], [IHBCE] [1]
Note(s): 1PhoB inhibits the clpS expression under low cytoplasmic Mg2+, and the resulting impact on abundance of a ClpSAP substrate is conserved between E. coli and Salmonella Yeom J,2018.1PhoB inhibits the clpS expression under low cytoplasmic Mg2+, and the resulting impact on abundance of a ClpSAP substrate is conserved between E. coli and Salmonella Yeom J,2018.


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -9.2 922866 922883 tcttgtcagcGGCAGGGCGTTCTGCCGataaccgtaa
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [IC] Inferred by curator

 [BCE] Binding of cellular extracts

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [GEA] Gene expression analysis

 [IHBCE] Inferred by a human based on computational evidence


Reference(s)    

 [1] Yeom J., Gao X., Groisman EA., 2018, Reduction in adaptor amounts establishes degradation hierarchy among protease substrates., Proc Natl Acad Sci U S A 115(19):E4483-E4492

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