RegulonDB RegulonDB 10.9: Operon Form
   

mreBCD operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mreBCD
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mreB
Gene(s): mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mreB gene appears to be positively regulated by FlhDC Pruss BM,2001 but it is not known which of the three promoters transcribing the gene is affected by the protein.
A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for mreB . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp1
+1: 3401129
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: ttcaccgtagcagatgatttttgcgccttgtcgctgctgcgtgtggttggtaaagtaagcGgattttcttttccgccccag
                            -35                    -10      +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[1] Wachi M., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: cgtatcagacCAGGCAGGGTAAACAGACACTTCCCCTGCCTGcatccgatta
Reference(s): [1] Wachi M., et al., 2006
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote BolA-phosphorylated repressor mreBp1 3401317 3401324 -191.5 tgcttgtctgCTCGCCAGAttgttgcagc nd [BPP], [GEA] [3], [4]


Transcription unit          
Name: mreBCD
Gene(s): mreD, mreC, mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on sequence analysis, 1937035 suggested that this transcription unit was shaped by five genes, mreBCD-yhdE-rng. However, Wachi M,2006demonstrated through Northern blot and gene expression analyses that this operon has three genes only, mreBCD. This operon appears to be positively regulated by FlhDC Pruss BM,2001 but it is not known which of the three promoters transcribing the operon is affected by the protein.
Evidence: [CV(LTED/PM)] cross validation(LTED/PM)
[LTED] Length of transcript experimentally determined
[PM] Polar mutation
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp1
+1: 3401129
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: ttcaccgtagcagatgatttttgcgccttgtcgctgctgcgtgtggttggtaaagtaagcGgattttcttttccgccccag
                            -35                    -10      +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[1] Wachi M., et al., 2006
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote BolA-phosphorylated repressor mreBp1 3401317 3401324 -191.5 tgcttgtctgCTCGCCAGAttgttgcagc nd [BPP], [GEA] [3], [4]


Transcription unit       
Name: mreBCD
Gene(s): mreD, mreC, mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on sequence analysis, 1937035 suggested that this transcription unit was shaped by five genes, mreBCD-yhdE-rng. However, Wachi M,2006demonstrated through Northern blot and gene expression analyses that this operon has three genes only, mreBCD. This operon appears to be positively regulated by FlhDC Pruss BM,2001 but it is not known which of the three promoters transcribing the operon is affected by the protein.
Evidence: [CV(LTED/PM)] cross validation(LTED/PM)
[LTED] Length of transcript experimentally determined
[PM] Polar mutation
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp2
+1: 3401193
Distance from start of the gene: 106
Sequence: gatgcattaagcctttctggactcaggcagagatttgtaacaaaggaaacgaactgcactAattttcaccgtagcagatga
Evidence: [TIM]
Reference(s): [1] Wachi M., et al., 2006


Transcription unit       
Name: mreB
Gene(s): mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mreB gene appears to be positively regulated by FlhDC Pruss BM,2001 but it is not known which of the three promoters transcribing the gene is affected by the protein.
A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for mreB . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp2
+1: 3401193
Distance from start of the gene: 106
Sequence: gatgcattaagcctttctggactcaggcagagatttgtaacaaaggaaacgaactgcactAattttcaccgtagcagatga
Evidence: [TIM]
Reference(s): [1] Wachi M., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: cgtatcagacCAGGCAGGGTAAACAGACACTTCCCCTGCCTGcatccgatta
Reference(s): [1] Wachi M., et al., 2006


Transcription unit       
Name: mreBCD
Gene(s): mreD, mreC, mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Based on sequence analysis, 1937035 suggested that this transcription unit was shaped by five genes, mreBCD-yhdE-rng. However, Wachi M,2006demonstrated through Northern blot and gene expression analyses that this operon has three genes only, mreBCD. This operon appears to be positively regulated by FlhDC Pruss BM,2001 but it is not known which of the three promoters transcribing the operon is affected by the protein.
Evidence: [ITCR] Inferred through co-regulation
[LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp3
+1: 3401356
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 269
Sequence: atattcacaaagatactcggtttaacctgccgtttaatccgttttcacgtagaataatgcGcgctgcgtctcatgggagtg
                           -35                    -10       +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[1] Wachi M., et al., 2006


Transcription unit       
Name: mreB
Gene(s): mreB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mreB gene appears to be positively regulated by FlhDC Pruss BM,2001 but it is not known which of the three promoters transcribing the gene is affected by the protein.
A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for mreB . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wachi M., et al., 2006
Promoter
Name: mreBp3
+1: 3401356
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 269
Sequence: atattcacaaagatactcggtttaacctgccgtttaatccgttttcacgtagaataatgcGcgctgcgtctcatgggagtg
                           -35                    -10       +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[1] Wachi M., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: cgtatcagacCAGGCAGGGTAAACAGACACTTCCCCTGCCTGcatccgatta
Reference(s): [1] Wachi M., et al., 2006


Transcription unit       
Name: mreCD
Gene(s): mreD, mreC   Genome Browser M3D Gene expression COLOMBOS
Evidence: [PAGTSBP] Products of adjacent genes in the same biological process
Promoter
Name: mreCp
+1: Unknown
Note(s): Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity.
Evidence: [IEP]
Reference(s): [5] Zaslaver A., et al., 2006





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