Name: |
rpsB-tsf |
Gene(s): |
tff, rpsB, tsf
Genome Browser
M3D Gene expression
COLOMBOS
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Note(s): |
There is a typical rho-independent termination or attenuation site in the intergenic region of rpsB and tsf which is preceded by a very stable stem-loop structure that, in its RNA form, is reminiscent of an RNA polymerase III site An G,1981. However, neither an attenuator effect nor promoter activity was observed An G,1981. |
Reference(s): |
[1] An G., et al., 1981
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Promoter |
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Name: |
rpsBp |
+1: |
189712 |
Sigma Factor: |
Sigma70 Sigmulon |
Distance from start of the gene: |
0
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Sequence: |
tgctgtacacagcgccaacaattattggtgtccacgacgtatttgtggtataaagcgcgcCggacttccgatccatttcgt -35 -10 +1
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Note(s): |
The rpsB promoter belongs to the extended -10 promoter class Aseev LV,2008 It comprises a TGTG extension upstream of the -10 hexamer TATAAA, a suboptimal -35 box, TTGGTG, and a GC-rich discriminator, GCGCGC, that separates the -10 element from the transcription start site and is reminiscent of a GC-rich discriminator typical for promoters under growth rate and stringent control Aseev LV,2014. Aseev LV,2008and references therein|. The design of the rpsB promoter region is highly conserved in γ-proteobacteria Aseev LV,2008 Site-directed mutation on both the TGTG extension and the TTG element in the -35 region had effects on transcription, since they dramatically decreased the activity of the promoter Aseev LV,2014 |
Evidence: |
[COMP-AINF] [COMP-HINF] [EXP-IDA-HPT-TRANSCR-INIT-M-RACE-MAP] [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] [RS-EPT-CBR]
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Reference(s): |
[2] Aseev LV., et al., 2008 [3] Huerta AM., et al., 2003 [4] Mendoza-Vargas A., et al., 2009 [5] Salgado H, et al., 2012
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Terminator(s) |
|
Type: |
rho-independent |
Sequence: |
atcaaaaaggAGCCGCCTGAGGGCGGCTtctttttgtg |
Reference(s): |
[1] An G., et al., 1981
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