RegulonDB RegulonDB 11.1: Operon Form
   

torR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: torR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: torR
Gene(s): torR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [1] Ansaldi M., et al., 2000
Promoter
Name: torRp
+1: 1057984
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 30
Sequence: aatgagcaaatatgaacagcggcactggtcaggatgaacggcttacggcagaatatgaacAgatatgaacagaatgagtaa
                               -35                    -10   +1                   
Evidence: [COMP-AINF]
[COMP-HINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Ansaldi M., et al., 2000
[2] Huerta AM., et al., 2003
[3] Simon G., et al., 1994
TF binding sites (TFBSs)
[TorR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal TorR repressor torRp 1057973 1057982 7.5 atatgaacagATATGAACAGaatgagtaaa nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-GSELEX], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [1], [4], [5]
proximal TorR repressor torRp 1057983 1057992 -3.5 ttacggcagaATATGAACAGatatgaacag nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-GSELEX], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] C [1], [4], [5]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF] Inferred by a human based on computational evidence

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-GSELEX] high throughput Genomic SELEX-chip evidence

 [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Ansaldi M., Simon G., Lepelletier M., Mejean V., 2000, The TorR high-affinity binding site plays a key role in both torR autoregulation and torCAD operon expression in Escherichia coli., J Bacteriol 182(4):961-6

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Simon G., Mejean V., Jourlin C., Chippaux M., Pascal MC., 1994, The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes., J Bacteriol 176(18):5601-6

 [4] Shimada T., Ogasawara H., Ishihama A., 2018, Single-target regulators form a minor group of transcription factors in Escherichia coli K-12., Nucleic Acids Res 46(8):3921-3936

 [5] Simon G., Jourlin C., Ansaldi M., Pascal MC., Chippaux M., Mejean V., 1995, Binding of the TorR regulator to cis-acting direct repeats activates tor operon expression., Mol Microbiol 17(5):971-80

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