RegulonDB RegulonDB 10.9: Operon Form
   

mgtLA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mgtLA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: mgtA
Gene(s): mgtL, mgtA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The mRNA that contains the mgtA gene has been observed mainly in the poles of the cell 31540875.
Promoter
Name: mgtAp2
+1: 4467280
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 151
Sequence: atctttgatggtcagccgattttgcatcctgttgtcctgtaacgtgttgtttaattatttGagcctaacgttacccgtgca
                                -35                  -10    +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[2] Yamamoto K., et al., 2002


Transcription unit          
Name: mgtA
Gene(s): mgtL, mgtA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The transcription of mgtA appears to be increased under acidic growth conditions during the exponential phase in a RcsB-dependent manner, but not during stationary phase Johnson MD,2011 However, it is not known which of the two promoters that transcribe this gene is affected by RcsB.
The mRNA that contains the mgtA gene has been observed mainly in the poles of the cell 31540875.
Promoter
Name: mgtAp1
+1: 4467363
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 68
Sequence: cagcaatgggtaaagtctggtttatcgttggtttagttgtcagcaggtattatatcgccaTagatgctacgaatattattg
                      -35                       -10         +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [1] Huerta AM., et al., 2003
[3] Kato A., et al., 1999
[4] Minagawa S., et al., 2003
[2] Yamamoto K., et al., 2002
TF binding sites (TFBSs)
[PhoP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoP-Phosphorylated activator mgtAp1 4467321 4467337 -34.0 ggtaaagtctGGTTTATCGTTGGTTTAGttgtcagcag nd [APIORCISFBSCS], [BPP], [GEA], [SM] [2], [3], [4], [5]


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [SM] Site mutation


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Yamamoto K., Ogasawara H., Fujita N., Utsumi R., Ishihama A., 2002, Novel mode of transcription regulation of divergently overlapping promoters by PhoP, the regulator of two-component system sensing external magnesium availability., Mol Microbiol 45(2):423-38

 [3] Kato A., Tanabe H., Utsumi R., 1999, Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters., J Bacteriol 181(17):5516-20

 [4] Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R., 2003, Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli., J Bacteriol 185(13):3696-702

 [5] Oshima T., Aiba H., Masuda Y., Kanaya S., Sugiura M., Wanner BL., Mori H., Mizuno T., 2002, Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12., Mol Microbiol 46(1):281-91

 [6] null, null, null, null


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