RegulonDB RegulonDB 10.9: Operon Form

arfA operon and associated TUs in Escherichia coli K-12 genome

Name: arfA
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit       
Name: arfA
Gene(s): arfA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The hairpin structure shown here as a terminator is in fact not a transcriptional terminator, but the site of RNase III cleavage of the mRNA Garza-Sanchez F,2011.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[ICWHO] Inferred computationally without human oversight
[LTED] Length of transcript experimentally determined
Reference(s): [1] Chadani Y., et al., 2011
Name: arfAp3
+1: 3438673
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 24
Sequence: gggcgagtggcgttaagagtggttgttgattttttgcactggcaggattacactcgcgccGttaaataaccaactggagtt
                          -35                    -10        +1                   
Note(s): Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.966. P-value: 1.225e-5
Evidence: [ICWHO]
Reference(s): [1] Chadani Y., et al., 2011
[2] Huerta AM., et al., 2003
Type: rho-independent
Reference(s): [3] Garza-Sanchez F., et al., 2011

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -11.1 3438761 3438792 ttaacgatgcCTGTTGTGGGACTGCTCATAGCAGTGTTTATtgttcgattc
  anti-terminator -7.8 3438780 3438827 cgagttgcagAGTATGCAGCGTTCCTTGCAACGCCTTAACGATGCCTGTTGTGGGACtgctcatagc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"