RegulonDB RegulonDB 11.1: Operon Form

cra operon and associated TUs in Escherichia coli K-12 genome

Name: cra
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: cra
Gene(s): cra   Genome Browser M3D Gene expression COLOMBOS
Note(s): Under nitrogen-rich growth conditions, the expression of the fruR gene was decreased in mutants for two genes that encode two terminal oxidases, cyoA and cydB, and increased in a mutant for the transcriptional regulator Fur. However, under nitrogen-limited growth conditions, the gene expression was increased in the cyoA and cydB mutants. It is unknown if the effects of Fur act directly on the gene expression Kumar R,2011
The expression of the gene cra is decreased under acidic growth conditions in either aerobiosis or microaerobiosis Marzan LW,2013 The decreased expression under aerobiosis appears to be caused by the transcription factor PhoB and also could be caused by the increased glucose concentration observed under acidic conditions Marzan LW,2013
Evidence: [COMP-AINF] Inferred computationally without human oversight
Name: fruRp8
+1: 87947
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 81
Sequence: cgttgggctttttttgcgaaatcagtgggaacctggaataaaagcagttgccgcagttaaTtttctgcgcttagatgttaa
                                 -35               -10      +1                   
Evidence: [COMP-AINF]
Reference(s): [1] Huerta AM., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd PhoB-phosphorylated repressor fruRp8 nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS] W [2]

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -12.4 87873 87902 gcaatttacaGCCCAACATGTCACGTTGGGCTTTTTTTGcgaaatcagt
  anti-terminator -17.1 87828 87876 ctttcgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCcaacatgtca
  anti-anti-terminator -10.9 87800 87832 ggaggttgctCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGAtaaaataatg
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"