RegulonDB RegulonDB 10.8: Operon Form
   

mcaS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mcaS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mcaS
Synonym(s): isrA
Gene(s): mcaS   Genome Browser M3D Gene expression COLOMBOS
Note(s): The RNA produced by the mcaS gene has been observed mainly in the poles of the cell 31540875.
Evidence: [AISDTU] Automated inference that a single-gene directon is a transcription unit
[LTED] Length of transcript experimentally determined
Reference(s): [1] Thomason MK., et al., 2012
Promoter
Name: mcaSp
+1: 1405751
Distance from start of the gene: 0
Sequence: cgtgaaatctgtcactgaagaaaattggcaactaaaggttaaaaccgttataacacagtcAccggcgcagaggagacaatg
Evidence: [TIM]
Reference(s): [1] Thomason MK., et al., 2012
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator mcaSp 1405793 1405815 -52.5 gtaacttataTTTTCGTGAAATCTGTCACTGAAgaaaattggc nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [1]


Evidence    

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(CHIP-SV/GEA/ROMA)] cross validation(CHIP-SV/GEA/ROMA)

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [GEA] Gene expression analysis


Reference(s)    

 [1] Thomason MK., Fontaine F., De Lay N., Storz G., 2012, A small RNA that regulates motility and biofilm formation in response to changes in nutrient availability in Escherichia coli., Mol Microbiol 84(1):17-35

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