RegulonDB RegulonDB 10.8: Operon Form
   

appCBXA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: appCBXA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: appCBA-yccB
Synonym(s): OP00137, appCBA, cyx-appA, cyxAB-appA
Gene(s): appC, appB, appX, appA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ITC] Inferred through co-regulation
[PM] Polar mutation
Reference(s): [1] Atlung T., et al., 1994
[2] Dassa J., et al., 1991
Promoter
Name: appCp
+1: 1037710
Sigma Factor: Sigma70, Sigma38
Distance from start of the gene: 30
Sequence: ggcgaatacctctggtcgtagagtttcaggataaagagggagatctaccattatcgggttAtttttctctcttcgcctaca
                        -35              -10                +1                   
Evidence: [HIPP]
Reference(s): [1] Atlung T., et al., 1994
[3] Atlung T., et al., 1997
[2] Dassa J., et al., 1991
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd AppY1 activator appCp nd nd nd nd nd [GEA] [1], [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd ArcA-Phosphorylated activator appCp nd nd nd nd nd [GEA] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal YdeO activator appCp 1037672 1037694 -27.5 tggtcgtagaGTTTCAGGATAAAGAGGGAGATCtaccattatc nd [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA], [IHBCE] [4]
Note(s): 1The AppY protein is also involved in anaerobic induction of the hya operon, which encodes hydrogenase 1 and is located immediately upstream from the appCBA operon. The AppY protein regulates AppA synthesis by activating transcription from the appCp promoter. Furthermore, the appY gene might be autoregulated. Genetic analysis of the appCp promoter region indicates that the presence of sequences upstream from the -10 region are required for activation of the appCp promoter by AppY protein and anaerobiosis, suggesting that this region has an AppY-binding site.2The AppY protein is also involved in anaerobic induction of the hya operon, which encodes hydrogenase 1 and is located immediately upstream from the appCBA operon. The AppY protein regulates AppA synthesis by activating transcription from the appCp promoter. Furthermore, the appY gene might be autoregulated. Genetic analysis of the appCp promoter region indicates that the presence of sequences upstream from the -10 region are required for activation of the appCp promoter by AppY protein and anaerobiosis, suggesting that this region has an AppY-binding site.


Transcription unit       
Name: appA
Gene(s): appA   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Atlung T., et al., 1994
Promoter
Name: appAp
+1: 1040599
Distance from start of the gene: 18
Sequence:
Evidence: [HIPP]
[TIM]
Reference(s): [2] Dassa J., et al., 1991





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