RegulonDB RegulonDB 11.1:Regulon Page

HyfR DNA-binding transcriptional activator

Synonyms: HyfR
HyfR , "hydrogenase four regulator," controls the expression of genes responsible for the proton-translocating formate hydrogenase system and for formate transport under anaerobiosis, low pH, and post-exponential growth conditions [3] As a member of the group of σ54-dependent transcriptional regulators, HyfR contains three domains: the sensory domain, located in the N terminus; the central domain, involved in open complex formation, ATP hydrolysis, and interaction with σ54, and the C-terminal domain, which contains a helix-turn-helix motif for DNA binding [3] An iron-sulfur cluster and a metal cofactor (possibly a similar metal or formate) have been proposed as the signals sensed by HyfR [2] HyfR shows 46% identity to FhlA, a member of the same group. These proteins are dissimilar in their N-terminal regions, which in FhlA recognizes formate. However, they show only one different residue in their helix-turn-helix motif, suggesting that they recognize similar DNA-binding sites [3] The HyfR protein is autoregulated.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
HyfR Functional   nd nd nd
Evolutionary Family: EBP
TFBs length: 18
TFBs symmetry: inverted-repeat
Connectivity class: Local Regulator
Gene name: hyfR
  Genome position: 2611900-2613912
  Length: 2013 bp / 670 aa
Operon name: hyfABCDEFGHIJR-focB
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) focB, hyfA, hyfB, hyfC, hyfD, hyfE, hyfF, hyfG, hyfH, hyfI, hyfJ, hyfR
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
anaerobic respiration (10)
membrane (6)
Porters (Uni-, Sym- and Antiporters) (1)
Transcription related (1)
activator (1)
Regulated operon(s) hyfABCDEFGHIJR-focB
First gene in the operon(s) hyfA
Simple and complex regulons CRP,FNR,FhlA,HyfR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
  HyfR activator hyfAp Sigma54 -129.5 -159.5 hyfA, hyfB, hyfC, hyfD, hyfE, hyfF, hyfG, hyfH, hyfI, hyfJ, hyfR, focB

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation