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Synonyms: Zur-Zn2+, Zur |
Summary:
Zur, "zinc uptake regulator," regulates genes involved in the Zn2+ ABC transporter uptake system and genes involved in the expression of alternative ribosomal proteins [2, 5, 7] The active conformation of Zur is observed when it is in a reduced form and when it is bound to Zn(II). The oxidized form of Zur does not bind to DNA or zinc. [2, 7, 10] Zur is not autoregulated [10] Zur, which belongs to the Fur family [7], appears to have a DNA-binding site in the N-terminal domain, around residue 65, and a signal-recognition site in the C-terminal domain. This protein has two Zn(II)-binding sites: site A, which only binds to Zn(II), and site B (the sensor site), which binds to Zn(II) and Co(II) with high and low affinities, respectively [1] Zur is able to form dimers, even in the absence of zinc [2]. The 23-bp palindrome consensus sequence for Zur-binding sites of gamma-proteobacteria, including Escherichia coli, has been determined by computational analysis [5] The thermodynamic profile of Zur-DNA interactions directly correlates with the physiological response at different promoters [9] Tyr45 and Arg65 interactions with Zur-box purines define the recognition motif [9] Zurs unbinding rate from DNA is sensitive to Zur concentration in a biphasic way; this was the first reporting of such a biphasic manner [11] These kinds of mechanisms could facilitate transcription switching between repression and derepression [11] A zur mutant enhanced cell survival when cells were exposed to biocides [12]. Read more > |
Transcription factor | ![]() ![]() |
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TF conformation(s): |
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Evolutionary Family: | Fur | |||||||||||||||||||||
TFBs length: | 23 | |||||||||||||||||||||
Connectivity class: | Local Regulator | |||||||||||||||||||||
Gene name: | zur | |||||||||||||||||||||
Genome position: | 4259488-4260003 | |||||||||||||||||||||
Length: | 516 bp / 171 aa | |||||||||||||||||||||
Operon name: | zur | |||||||||||||||||||||
TU(s) encoding the TF: |
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Regulon |
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Regulated gene(s) | ykgM, ykgO, zinT, znuA, znuB, znuC | ||||||
Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
ribosomal proteins (2)
adaptations (2)
ABC superfamily, periplasmic binding component (1)
ABC superfamily, membrane component (1)
membrane (1)
Read more >
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Regulated operon(s) | ykgMO, zinT, znuA, znuCB | ||||||
First gene in the operon(s) | ykgM, zinT, znuA, znuC | ||||||
Simple and complex regulons | Fur,OxyR,SoxS,Zur OxyR,Zur Zur | ||||||
Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) |
Transcription factor regulation |
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Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | References | |
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Zur-Zn2+ | repressor | ykgMp | Sigma70 | 11.0 | -18.0 | ykgM, ykgO |
gtatctttatGGTTATGTTATAACATAACAGGTAaaaatgatga
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312784 | 312807 | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS], [COMP-HINF-SIMILAR-TO-CONSENSUS] | W | [3], [4], [5] | |
Zur-Zn2+ | repressor | zinTp | Sigma70 | -16.0 | -45.0 | zinT |
tgcattttgcTATATGTTACAATATAACATTACacatcatata
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2041319 | 2041341 | [COMP-AINF-SIMILAR-TO-CONSENSUS] | nd | [5] | |
Zur-Zn2+ | repressor | znuAp | Sigma38 | 38.0 | -41.0 | znuA |
gcagtagtcaTGAAATGTTATAATATCACACTTCtcatattcat
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1942613 | 1942636 | [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | S | [2], [6], [7], [8] | |
Zur-Zn2+ | repressor | znuCp | Sigma70 | -10.0 | -38.0 | znuC, znuB |
atgaatatgaGAAGTGTGATATTATAACATTTCAtgactactgc
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1942613 | 1942636 | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | W | [2], [6], [7] |
Functional conformation | Function | Object name | Object type | Distance to first Gene | Sequence | LeftPos | RightPos | Center Position | Growth Condition | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | References | |
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Zur-Zn2+ | repressor | pliG | Transcription-Unit | nd |
gttcagaatgAGTACGATGAACTGTTATAATATAacaatcccta
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1227495 | 1227518 | 1227506.0 | nd | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] | W | [9] |
Evolutionary conservation of regulatory elements | ![]() |
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Reference(s) |
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