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IlvY DNA-binding transcriptional dual regulator

Synonyms: IlvY-(S)-2-acetolactate, IlvY, IlvY-Alfa-acetohydroxybutirate
Summary:
In the presence of α-acetolactate or α-acetohydroxybutyrate, the "isoleucine-valine" protein, IlvY, participates in activating a gene involved in isoleucine and valine biosynthesis. In the absence of the metabolites, it is autorepressed [2, 5]. IlvY binds cooperatively to two inverted repeat DNA-binding sites of 21 bp in the divergent regulatory region of the ilvY and ilvC genes for autorepression. When the inducer is bound to the IlvY-DNA complex, IlvY binding at the same DNA position activates the transcription of ilvC by inducing a conformational change in the structure of the complex that is correlated with the relief of a protein-induced bend in the DNA that enhances the recruitment of RNA polymerase [2, 5]. The transcription of the divergent genes ilvY and ilvC is coupled in a DNA supercoiling-dependent manner [7]. The protein belongs to the LysR family and contains a helix-turn-helix motif for DNA binding near its N terminus [6]. Read more >


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
IlvY Functional   Apo [BPP], [IDA], [IPI], [SM] [1], [2], [3], [4], [5]
IlvY-(S)-2-acetolactate Functional Allosteric Holo [AIFS], [BPP], [IDA], [IPI], [SM] [2], [5], [6]
IlvY-Alfa-acetohydroxybutirate Functional Allosteric Holo [BPP], [IDA], [IPI], [SM] [2]
Evolutionary Family: LysR
Sensing class: Using internal synthesized signals
Connectivity class: Local Regulator
Gene name: ilvY
  Genome position: 3956927-3957820
  Length: 894 bp / 297 aa
Operon name: ilvY
TU(s) encoding the TF:
Transcription unit        Promoter
ilvY
ilvYp1
ilvY
ilvYp2


Regulon       
Regulated gene(s) ilvC, ilvY
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
isoleucine/valine (2)
Transcription related (1)
activator (1)
repressor (1)
operon (1)
Regulated operon(s) ilvC, ilvY
First gene in the operon(s) ilvC, ilvY
Simple and complex regulons IlvY
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[IlvY,+](1)
[IlvY,-](2)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  IlvY-(S)-2-acetolactate activator ilvCp Sigma70 -61.0 -119.0 ilvC
cgatgttgacGTTGCAAAAATTGCAATGtgacgttgtg
3957843 3957860 [APIORCISFBSCS], [BCE], [BPP], [GEA], [IC] [2], [5]
  IlvY-(S)-2-acetolactate activator ilvCp Sigma70 -30.0 -88.0 ilvC
cgttgtgaatATATCAATTTCCGCAATAaatttcctgt
3957874 3957891 [APIORCISFBSCS], [BCE], [BPP], [GEA], [IC] [2], [5]
  IlvY repressor ilvYp1 Sigma70 -15.0 -62.0 ilvY
acaggaaattTATTGCGGAAATTGATATattcacaacg
3957874 3957891 [APIORCISFBSCS], [BCE], [BPP], [GEA], [IC] [2], [5]
  IlvY repressor ilvYp1 Sigma70 17.0 -31.0 ilvY
cacaacgtcaCATTGCAATTTTTGCAACgtcaacatcg
3957843 3957860 [APIORCISFBSCS], [BCE], [BPP], [GEA], [IC] [2], [5]
  IlvY repressor ilvYp2 Sigma38 -15.0 -62.0 ilvY
acaggaaattTATTGCGGAAATTGATATattcacaacg
3957874 3957891 [APIORCISFBSCS], [BCE], [BPP], [GEA], [IC] [2], [5]
  IlvY repressor ilvYp2 Sigma38 17.0 -31.0 ilvY
cacaacgtcaCATTGCAATTTTTGCAACgtcaacatcg
3957843 3957860 [APIORCISFBSCS], [BCE], [BPP], [GEA], [IC] [2], [5]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation






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