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PepA DNA-binding transcriptional repressor

Synonyms: PepA
Aminopeptidase A/I is a Peptidase that binds DNA and controls the transcription of the operon involved in the synthesis of carbamoyl phosphate, which is an intermediate related to the pyrimidine nucleotide pathway [1, 2, 3]. This protein also is required for maintenance of plasmid monomers, which is critical for proper plasmid segregation [4]. Aminopeptidase A/I is a part of the site-specific recombination system required to decatenate the plasmids ColE1 and pSC101, maintaining them as monomers []. Aminopeptidase A/I is responsible for coordinating DNA strands during recombination, pairing cer sites in ColE1 plasmids and allowing the formation of Holliday junctions []. Aminopeptidase A/I, along with ArgR, may be involved in blocking trans-recombination, thus preventing the formation of new catenated plasmids during recombination [].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
PepA Functional   nd [1]
Evolutionary Family: Aminopeptidase
Connectivity class: Local Regulator
Gene name: pepA
  Genome position: 4484440-4485951
  Length: 1512 bp / 503 aa
Operon name: pepA
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) carA, carB, pepA
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
arginine (2)
pyrimidine biosynthesis (2)
proteins/peptides/glycopeptides (1)
DNA recombination (1)
Transcription related (1)
Regulated operon(s) carAB, pepA
First gene in the operon(s) carA, pepA
Simple and complex regulons Fis,IHF,PepA,PurR,RutR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  PepA repressor carAp1 Sigma70 -173.0 -273.0 carA, carB
29365 29391 [BPP], [GEA], [IMP] [2]
  PepA repressor carAp1 Sigma70 -79.0 -179.0 carA, carB
29459 29485 [BPP], [GEA] [1], [2]
  PepA repressor pepAp1 Sigma70 -43.0 -231.0 pepA
4486169 4486195 [BPP] [3]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation