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BolA DNA-binding transcriptional dual regulator

Synonyms: BolA-phosphorylated
Summary:
The heterodimeric Grx4-BolA complex can bind an intact [2Fe-2S] cluster and transfer it to the model acceptor protein ferredoxin, although much less efficiently than the Grx4 homodimer [6].


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
BolA-phosphorylated Functional   nd nd
Evolutionary Family: BolA
TFBs length: 8
TFBs symmetry: inverted-repeat
Sensing class: External-Two-component systems
Connectivity class: Local Regulator
Gene name: bolA
  Genome position: 454472-454789
  Length: 318 bp / 105 aa
Operon name: bolA
TU(s) encoding the TF:
Transcription unit        Promoter
bolA
bolAp
bolA
bolAp1
bolA
bolAp2


Regulon       
Regulated gene(s) ampC, csgA, csgD, csgE, csgF, csgG, dacC, mreB, mreC, mreD
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
murein (peptidoglycan) (2)
fimbri, pili (2)
membrane (2)
biosynthesis of macromolecules (cellular constituents) (2)
drug resistance/sensitivity (1)
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Regulated operon(s) ampC, csgBAC, csgDEFG, dacC, mreBCD
First gene in the operon(s) ampC, csgA, csgD, dacC, mreB
Simple and complex regulons BasR,BolA,BtsR,CRP,CpxR,Cra,CsgD,FliZ,H-NS,IHF,MlrA,MqsA,OmpR,RcdA,RcsAB,RstA,ppGpp
BolA
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[BolA,-](3)
[BolA,+](4)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  BolA-phosphorylated activator ampCp Sigma70 nd nd ampC
nd
 nd nd [GEA], [IMP], [BPP] [1]
  BolA-phosphorylated repressor ampCp Sigma70 nd nd ampC
nd
 nd nd [GEA], [IMP], [BPP] [1]
  BolA-phosphorylated activator csgAp Sigma70 nd nd csgA
nd
 nd nd [GEA], [BPP] [2]
  BolA-phosphorylated activator csgDp3 Sigma38 nd nd csgD, csgE, csgF, csgG
nd
 nd nd [GEA], [BPP] [2]
  BolA-phosphorylated activator dacCp Sigma70 nd nd dacC
nd
 nd nd [GEA], [IMP], [BPP] [1], [3]
  BolA-phosphorylated repressor dacCp Sigma70 nd nd dacC
nd
 nd nd [GEA], [IMP], [BPP] [1], [3]
  BolA-phosphorylated repressor mreBp1 Sigma70 -191.5 -233.5 mreB, mreC, mreD
gcttgtctgcTCGCCAGAttgttgcagc
 3401317 3401324 [GEA], [BPP] [4], [5]



High-throughput Transcription factor binding sites (TFBSs)
      

  Functional conformation Function Object name Object type Distance to first Gene Sequence LeftPos RightPos Center Position Growth Condition Evidence (Confirmed, Strong, Weak) References
  BolA-phosphorylated activator fimH Gene nd
nd
nd nd nd nd [GEA], [BPP] [2]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [GEA] Gene expression analysis

 [IMP] Inferred from mutant phenotype

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Santos JM., Lobo M., Matos AP., De Pedro MA., Arraiano CM., 2002, The gene bolA regulates dacA (PBP5), dacC (PBP6) and ampC (AmpC), promoting normal morphology in Escherichia coli., Mol Microbiol 45(6):1729-40

 [2] Azam MW., Zuberi A., Khan AU., 2020, bolA gene involved in curli amyloids and fimbriae production in E. coli: exploring pathways to inhibit biofilm and amyloid formation., J Biol Res (Thessalon) 27:10

 [3] Guinote IB., Matos R., Freire P., Arraiano C., 2011, BolA Affects Cell Growth, and Binds to the Promoters of Penicillin-Binding Proteins 5 and 6 and Regulates Their Expression., J Microbiol Biotechnol 21(3):243-51

 [4] Freire P., Moreira RN., Arraiano CM., 2009, BolA inhibits cell elongation and regulates MreB expression levels., J Mol Biol 385(5):1345-51

 [5] Galego L., Barahona S., Romao CV., Arraiano CM., 2020, Phosphorylation status of BolA affects its role in transcription and biofilm development., FEBS J

 [6] Yeung N, Gold B, Liu NL, Prathapam R, Sterling HJ, Willams ER, Butland G, 2011, The E. coli monothiol glutaredoxin GrxD forms homodimeric and heterodimeric FeS cluster containing complexes., Biochemistry, 50(41):8957 10.1021/bi2008883


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