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AlaS DNA-binding transcriptional repressor

Synonyms: AlaS, AlaS-L-alanine
Alanine—tRNA ligase (AlaRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. The reaction is driven by ATP hydrolysis. AlaRS belongs to the Class IIC aminoacyl tRNA synthetases []. AlaRS was seen as a homotetramer in solution []. Later experiments determined that AlaRS is homodimeric in solution [] and can exist in an equilibrium between a homodimeric and a homodecameric state, depending on temperature []. The enzyme contains one molecule of zinc per AlaS polypeptide []; zinc binds cooperatively and induces a conformational change in AlaRS [].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
AlaS Non-Functional   Apo [BPP] [1]
AlaS-L-alanine Functional Allosteric Holo [APPHINH], [BPP], [HIFS], [IEP] [1]
Evolutionary Family: Class II aminoacyl_tRNA synthetase
Connectivity class: Local Regulator
Gene name: alaS
  Genome position: 2819381-2822011
  Length: 2631 bp / 876 aa
Operon name: alaS
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) alaS
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
amino acid -activation (1)
repressor (1)
Regulated operon(s) alaS
First gene in the operon(s) alaS
Simple and complex regulons AlaS
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  AlaS-L-alanine repressor alaSp nd -6.5 -85.5 alaS
2822080 2822114 [BPP], [CV(CHIP-SV/SM)], [GEA], [SM] [1], [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation