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NemR DNA-binding transcriptional repressor

Synonyms: NemR-methylglyoxal, NemR
"N-ethylmaleimide reductase repressor," [2] represses the nemA gene, which is involved in reductive degradation of N-ethylmaleimide (NEM) and other toxic nitrous compounds, and it also represses its own expression. NemR belongs to the TetR family of HTH-type DNA-binding transcription factors [1, 3] NemR binds to a region 16 bp in length, and its consensus is a palindromic sequence, TAGACCnnnnGGTCTA, which is referred as the NemR box [2] This box sequence, TAGACCnnnnGGTCTA, is close to the recognition sequence, TTGACCAnnTGGTCAA, of RutR (the RutR box). RutR can bind upstream of the nemRA operon; however, it does not play an important role in regulation of nemRA expression in vivo [2] Presently, the nemRA operon is the unique member of the NemR regulon. Based on genomic SELEX and gene expression analyses, NemR could be affecting other genes, such as ydhF-ydhL, gloA, sapABCDF, yeaU, pck yeaT, and yhgE [2] however, the regulation of the genes under control of NemR requires more detailed studies before it can be solved. The NemRA system plays an important role in Escherichia coli survival in the presence of the toxic compound N-ethylmaleimide (NEM), which is the inducer for depression of the nemRA operon [2] It has been experimentally verified that methylglyoxal (MG) and not NEM is the real inducer for the nemRA operon [4] This induction was determined by ChIP-chip analysis with DNA-RNAP occupancy when cells were exposed to growth-inhibitory concentrations of MG [4] NemR is a transcriptional repressor that is sensitive to HOCl and related reactive chlorine species (RCS), and its gene products contribute to increased bleach resistance by detoxifying reactive electrophiles produced during this process [] The NemR regulator is controlled by alkylation of one or more of its Cys residues.
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
NemR Functional   Apo [APPH], [HIFS] [1], [2]
NemR-methylglyoxal Non-Functional   Holo nd nd
Evolutionary Family: TetR/AcrR
Connectivity class: Local Regulator
Gene name: nemR
  Genome position: 1726023-1726622
  Length: 600 bp / 199 aa
Operon name: nemRA-gloA
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) gloA, nemA, nemR
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
methylglyoxal metabolism (1)
unassigned reversible reactions (1)
detoxification (1)
transcriptional level (1)
Regulated operon(s) nemRA-gloA
First gene in the operon(s) nemR
Simple and complex regulons NemR,RutR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  NemR repressor nemRp Sigma70 -20.5 -22.5 nemR, nemA, gloA
1725993 1726009 [AIBSCS], [BPP], [GEA] [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation