RegulonDB RegulonDB 10.9:Regulon Page

DinJ-YafQ DNA-binding transcriptional repressor

Synonyms: DinJ-YafQ
The YafQ-DinJ toxin-antitoxin system was identified by its similarity to the RelE-RelB toxin-antitoxin system []. Expression of YafQ alone reduces protein synthesis and inhibits growth, and coexpression of DinJ alleviates that phenotype, acting as the antitoxin [1, 2]. YafQ and DinJ form a stable complex [1] which can bind to the dinJ-yafQ palindrome upstream of the translation start site [2]. A strain from which all five toxin-antitoxin systems have been deleted shows no deficiency in its stress response or competitiveness under nutrient-limited conditions []. However, biofilm formation is affected via expression of the TabA protein [].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
DinJ-YafQ Functional   [IDA], [IGI] [1], [2]
Connectivity class: Local Regulator
Gene name: dinJ
  Genome position: 246242-246502
  Length: 261 bp / 86 aa
Operon name: dinJ-yafQ
TU(s) encoding the TF:
Transcription unit        Promoter
Gene name: yafQ
  Genome position: 245961-246239
  Length: 279 bp / 92 aa
Operon name: dinJ-yafQ
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) cspE, dinJ, yafQ
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
cell killing (2)
transcriptional level (1)
Regulated operon(s) cspE, dinJ-yafQ
First gene in the operon(s) cspE, dinJ
Simple and complex regulons DinJ-YafQ
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  DinJ-YafQ repressor cspEp1 Sigma70 -43.5 -188.5 cspE
657096 657112 [BPP], [GEA], [SM] [3]
  DinJ-YafQ repressor dinJp Sigma70 89.5 -24.5 dinJ, yafQ
246519 246535 [APIORCISFBSCS], [BPP], [SM] [2]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation