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DgoR DNA-binding transcriptional repressor

Synonyms: DgoR-D-galactonate, DgoR
DgoR belongs to the FadR subfamily within the GntR family of transcriptional regulators, and its DNA-binding operator sequence is [5'-TTGTA(G/C)TACA(A/T)-3'] [3, 4]. The signature of GntR family regulators that also bind inverted repeats is [5'-(N)yGT(N)xAC(N)y-3'] [5]. There are three IRs (inverted repeats) in the regulation regions of the dgo genes. DgoR binds the IR1 and IR2 sites in the cis-acting element for repressing the dgoRKADT operon, and IR1 is essential for DgoR activity. The D7, L34, T40, R42, R46, and S51 amino acid residues are important for DgoR to bind its cis-acting element. On the other hand, the IR3 site is not critical for the interaction of DgoR with its cis-acting element [4]. The DgoR binding sites overlap with the promoter, repressing the transcription of the dgoRKADT operon by occluding the binding of RNA polymerase [4]. Based on a bioinformatics study, DgoR exists together with its binding sites in 13 genomes of different bacterial species, with the majority of members belonging to the family Enterobacteriaceae [5]. Production of the enzymes involved in D-galactonate degradation are induced by growth on galactonate [].
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Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence (Confirmed, Strong, Weak) References
DgoR Functional   Apo [APPPINH], [HIFS], [IDA], [IEP], [IPI] [1], [2], [3], [4]
DgoR-D-galactonate Non-Functional   Holo nd nd
Connectivity class: Local Regulator
Gene name: dgoR
  Genome position: 3874471-3875160
  Length: 690 bp / 229 aa
Operon name: dgoRKADT
TU(s) encoding the TF:
Transcription unit        Promoter

Regulated gene(s) dgoA, dgoD, dgoK, dgoR, dgoT
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (4)
Transcription related (1)
repressor (1)
operon (1)
Porters (Uni-, Sym- and Antiporters) (1)
Regulated operon(s) dgoRKADT
First gene in the operon(s) dgoR
Simple and complex regulons CRP,DgoR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)

Transcription factor regulation    

Transcription factor binding sites (TFBSs) arrangements

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence (Confirmed, Strong, Weak) References
  DgoR repressor dgoRp Sigma70 -110.0 -135.0 dgoR, dgoK, dgoA, dgoD, dgoT
3875290 3875301 [AIBSCS], [AIBSPD], [BPP], [GEA], [SM] [1], [4]
  DgoR repressor dgoRp Sigma70 -88.5 -113.5 dgoR, dgoK, dgoA, dgoD, dgoT
3875268 3875280 [AIBSCS], [AIBSPD], [BPP], [GEA], [SM] [1], [4]

Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation