RegulonDB RegulonDB 10.8: Regulon Form
   
   


CRP,CreB,Fis,MalT regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
Fis Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class TFs for DNA-bending
Synonym(s): DNA-binding transcriptional dual regulator Fis, Nbp, site-specific DNA inversion stimulation factor
Gene name(s): fis
Functional conformation(s): Fis
Coregulator(s): AcrR, AdiY, AppY, ArcA, ArgP, BasR, CRP, CpxR, Cra, CreB, CspA, CusR, CytR, DeoR, DksA, DksA-ppGpp, DnaA, FNR, FadR, Fis, FlhDC, FliZ, Fur, GadE, GadE-RcsB, GadW, GadX, GlcC, GlpR, H-NS, HprR, IHF, IscR, LeuO, Lrp, MalT, MarA, MarR, MazE, MazE-MazF, Mlc, MntR, ModE, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PdhR, PepA, PurR, RcsB, RcsB-BglJ, Rob, RstA, RutR, SlyA, SoxS, StpA, TorR, XylR, YdeO, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP , CRP-cAMP
Coregulator(s): AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YiaJ, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
MalT Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional activator MalT, MalA
Gene name(s): malT
Functional conformation(s): MalT-ADP-Maltotriose , MalT , MalT-Aes , MalT-MalK , MalT-MalY , MalT-maltotriose-ATP
Coregulator(s): CRP, CreB, FNR, Fis, MalT, PhoP
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
CreB      Tractordb tool
Connectivity class Local Regulator
Sensing Class External-Two-component systems
Synonym(s): CreB response regulator, DNA-binding transcriptional regulator CreB, YjjE
Gene name(s): creB
Functional conformation(s): CreB , CreB-Phosphorylated
Coregulator(s): CRP, CreB, Fis, MalT
Summary: TF Summary ...[more]

REGULATION EXERTED BY CRP,CreB,Fis,MalT Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
CRP activator CreB repressor Fis activator MalT activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
MalT-maltotriose-ATP activator malEp malG , malF , malE 4246667 4246676 -207.5 cttttttatgGGGGAGGAGGcgggaggatg [APIORCISFBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[1]
[2]
[3]
[4]
[5]
MalT-maltotriose-ATP activator malEp malG , malF , malE 4246657 4246666 -197.5 ggggaggaggCGGGAGGATGagaacacggc [AIBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[1]
[2]
[3]
[4]
[5]
CRP-cAMP activator malEp malG , malF , malE 4246625 4246646 -171.5 agaacacggcTTCTGTGAACTAAACCGAGGTCatgtaaggaa [AIBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[2]
[3]
[6]
CRP-cAMP activator malEp malG , malF , malE 4246593 4246614 -139.5 atgtaaggaaTTTCGTGATGTTGCTTGCAAAAatcgtggcga [APIORCISFBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[3]
[7]
[6]
CRP-cAMP activator malEp malG , malF , malE 4246559 4246580 -105.5 cgtggcgattTTATGTGCGCATCTCCACATTAccgccaattc [AIBSCS]
[APIORCISFBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[2]
[3]
[7]
[6]
CRP-cAMP activator malEp malG , malF , malE 4246530 4246551 -76.5 ttaccgccaaTTCTGTAACAGAGATCACACAAagcgacggtg [APIORCISFBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[8]
[3]
[7]
[6]
MalT-maltotriose-ATP activator malEp malG , malF , malE 4246691 4246700 -231.5 tggcttaaatCCTCCACCCCctggcttttt [APIORCISFBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[1]
[2]
[3]
[4]
[5]
MalT-maltotriose-ATP activator malEp malG , malF , malE 4246511 4246520 -51.5 aagcgacggtGGGGCGTAGGggcaaggagg [AIBSCS]
[APIORCISFBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[1]
[2]
[3]
[4]
[5]
MalT-maltotriose-ATP activator malEp malG , malF , malE 4246501 4246510 -41.5 ggggcgtaggGGCAAGGAGGatggaaagag [APIORCISFBSCS]
[BPP]
[CV(CHIP-SV/SM)]
[SM]
[1]
[2]
[3]
[4]
[5]
Fis activator malEp malG , malF , malE 4246571 4246590 -116.0 ttgcaaaaatCGTGGCGATTTTATGTGCGCatctccacat [AIBSCS]
[CV(GEA/ROMA)]
[GEA]
[9]
Fis activator malEp malG , malF , malE 4246404 4246423 52.0 aaggaccataGATTATGAAAATAAAAACAGgtgcacgcat [AIBSCS]
[CV(GEA/ROMA)]
[GEA]
[9]
CreB-Phosphorylated repressor malEp malG , malF , malE 4246438 4246456 18.5 ccgtttaggtGTTTTCACGAGCACTTCACcaacaaggac [AIBSCS]
[CV(GEA/ROMA)]
[GEA]
[10]

Evidence: [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[BPP] Binding of purified proteins
[CV(CHIP-SV/SM)] cross validation(CHIP-SV/SM)
[SM] Site mutation
[AIBSCS] Automated inference based on similarity to consensus sequences
[CV(GEA/ROMA)] cross validation(GEA/ROMA)
[GEA] Gene expression analysis
Reference(s): [1] Boos W., et al., 1998
[2] Raibaud O. 1989
[3] Raibaud O., et al., 1989
[4] Richet E. 1996
[5] Richet E., et al., 1991
[6] Vidal-Ingigliardi D., et al., 1991
[7] Richet E. 2000
[8] Merkel TJ., et al., 1992
[9] Bradley MD., et al., 2007
[10] Avison MB., et al., 2001



Reference(s)    

 [1] Boos W., Shuman H., 1998, Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation., Microbiol Mol Biol Rev 62(1):204-29

 [2] Raibaud O., 1989, Nucleoprotein structures at positively regulated bacterial promoters: homology with replication origins and some hypotheses on the quaternary structure of the activator proteins in these complexes., Mol Microbiol 3(3):455-8

 [3] Raibaud O., Vidal-Ingigliardi D., Richet E., 1989, A complex nucleoprotein structure involved in activation of transcription of two divergent Escherichia coli promoters., J Mol Biol 205(3):471-85

 [4] Richet E., 1996, On the role of the multiple regulatory elements involved in the activation of the Escherichia coli malEp promoter., J Mol Biol 264(5):852-62

 [5] Richet E., Raibaud O., 1991, Supercoiling is essential for the formation and stability of the initiation complex at the divergent malEp and malKp promoters., J Mol Biol 218(3):529-42

 [6] Vidal-Ingigliardi D., Raibaud O., 1991, Three adjacent binding sites for cAMP receptor protein are involved in the activation of the divergent malEp-malKp promoters., Proc Natl Acad Sci U S A 88(1):229-33

 [7] Richet E., 2000, Synergistic transcription activation: a dual role for CRP in the activation of an Escherichia coli promoter depending on MalT and CRP., EMBO J 19(19):5222-32

 [8] Merkel TJ., Nelson DM., Brauer CL., Kadner RJ., 1992, Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene., J Bacteriol 174(9):2763-70

 [9] Bradley MD., Beach MB., de Koning AP., Pratt TS., Osuna R., 2007, Effects of Fis on Escherichia coli gene expression during different growth stages., Microbiology 153(Pt 9):2922-40

 [10] Avison MB., Horton RE., Walsh TR., Bennett PM., 2001, Escherichia coli CreBC is a global regulator of gene expression that responds to growth in minimal media., J Biol Chem 276(29):26955-61


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