RegulonDB RegulonDB 10.9: Regulon Form
   
   


Ada regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
Ada      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional dual regulator / DNA repair protein Ada
Gene name(s): ada
Functional conformation(s): Ada , Ada-Methylated
Coregulator(s): Ada, AidB, Lrp
Summary: TF Summary ...[more]

REGULATION EXERTED BY Ada Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
Ada activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
Ada activator alkAp alkA 2147589 2147613 -41.5 gccgtcgcgaCAACCGGAATATGAAAGCAAAGCGCagcgtctgaa   [1]
[2]
[3]
[4]
[5]
[6]
Ada activator alkAp2 alkA 2147589 2147613 -41.5 gccgtcgcgaCAACCGGAATATGAAAGCAAAGCGCagcgtctgaa   [1]
[2]
[3]
[4]
[5]
[6]
Ada dual    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
Ada activator adap alkB , ada 2310474 2310498 -58.5 aaaagcttccTTGTCAGCGAAAAAAATTAAAGCGCaagattgttg   [7]
[8]
[9]
[3]
[4]
[10]
[5]
[11]
[6]
Ada repressor adap alkB , ada 2310454 2310478 -38.5 aaaaaattaaAGCGCAAGATTGTTGGTTTTTGCGTgatggtgacc   [9]
[3]
[4]
[5]
Ada repressor adap2 alkB , ada 2310454 2310478 -38.5 aaaaaattaaAGCGCAAGATTGTTGGTTTTTGCGTgatggtgacc   [9]
[3]
[4]
[5]
Ada activator adap2 alkB , ada 2310474 2310498 -58.5 aaaagcttccTTGTCAGCGAAAAAAATTAAAGCGCaagattgttg   [7]
[8]
[9]
[3]
[4]
[10]
[5]
[11]
[6]

Reference(s): [1] Furuichi M., et al., 1992
[2] Landini P., et al., 1997
[3] Saget BM., et al., 1995
[4] Saget BM., et al., 1994
[5] Sakumi K., et al., 1989
[6] Teo I., et al., 1986
[7] Landini P., et al., 1995
[8] Landini P., et al., 1995
[9] Nakamura T., et al., 1988
[10] Sakumi K., et al., 1993
[11] Taketomi A., et al., 1996



Reference(s)    

 [1] Furuichi M., Yu CG., Anai M., Sakumi K., Sekiguchi M., 1992, Regulatory elements for expression of the alkA gene in response to alkylating agents., Mol Gen Genet 236(1):25-32

 [2] Landini P., Gaal T., Ross W., Volkert MR., 1997, The RNA polymerase alpha subunit carboxyl-terminal domain is required for both basal and activated transcription from the alkA promoter., J Biol Chem 272(25):15914-9

 [3] Saget BM., Shevell DE., Walker GC., 1995, Alteration of lysine 178 in the hinge region of the Escherichia coli ada protein interferes with activation of ada, but not alkA, transcription., J Bacteriol 177(5):1268-74

 [4] Saget BM., Walker GC., 1994, The Ada protein acts as both a positive and a negative modulator of Escherichia coli's response to methylating agents., Proc Natl Acad Sci U S A 91(21):9730-4

 [5] Sakumi K., Sekiguchi M., 1989, Regulation of expression of the ada gene controlling the adaptive response. Interactions with the ada promoter of the Ada protein and RNA polymerase., J Mol Biol 205(2):373-85

 [6] Teo I., Sedgwick B., Kilpatrick MW., McCarthy TV., Lindahl T., 1986, The intracellular signal for induction of resistance to alkylating agents in E. coli., Cell 45(2):315-24

 [7] Landini P., Volkert MR., 1995, RNA polymerase alpha subunit binding site in positively controlled promoters: a new model for RNA polymerase-promoter interaction and transcriptional activation in the Escherichia coli ada and aidB genes., EMBO J 14(17):4329-35

 [8] Landini P., Volkert MR., 1995, Transcriptional activation of the Escherichia coli adaptive response gene aidB is mediated by binding of methylated Ada protein. Evidence for a new consensus sequence for Ada-binding sites., J Biol Chem 270(14):8285-9

 [9] Nakamura T., Tokumoto Y., Sakumi K., Koike G., Nakabeppu Y., Sekiguchi M., 1988, Expression of the ada gene of Escherichia coli in response to alkylating agents. Identification of transcriptional regulatory elements., J Mol Biol 202(3):483-94

 [10] Sakumi K., Igarashi K., Sekiguchi M., Ishihama A., 1993, The Ada protein is a class I transcription factor of Escherichia coli., J Bacteriol 175(8):2455-7

 [11] Taketomi A., Nakabeppu Y., Ihara K., Hart DJ., Furuichi M., Sekiguchi M., 1996, Requirement for two conserved cysteine residues in the Ada protein of Escherichia coli for transactivation of the ada promoter., Mol Gen Genet 250(5):523-32


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