RegulonDB RegulonDB 10.9: Regulon Form
   
   


CRP,H-NS,LeuO,SlyA,StpA regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP-cAMP , CRP
Coregulator(s): AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YiaJ, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
H-NS      Tractordb tool
Connectivity class Global Regulator
Synonym(s): BglY, Cur, DNA-binding protein HLP-II (HU, BH2, HD, NS), DNA-binding transcriptional dual regulator H-NS, Drc, DrdX, Drs, FimG, Hns, HnsA, Irk, MsyA, OsmZ, PilG, TopS, TopX, VirR
Gene name(s): hns
Functional conformation(s): H-NS
Coregulator(s): AcrR, AdiY, ArcA, ArgP, ArgR, BasR, BolA, BtsR, CRP, CadC, CaiF, CdaR, CpxR, Cra, CreB, CsgD, CspA, CysB, DksA, DksA-ppGpp, DnaA, DpiA, EvgA, FNR, Fis, FlhDC, FliZ, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GlaR, GutM, H-NS, HU, HdfR, HypT, IHF, LacI, LeuO, LrhA, Lrp, MalT, MarA, MatA, McbR, MlrA, MntR, MqsA, NagC, NanR, NarL, NarP, NhaR, NrdR, NsrR, OmpR, OxyR, PhoB, PhoP, QseB, RcdA, RcsAB, RcsB, RcsB-BglJ, Rob, RstA, RutR, SdiA, SlyA, SoxS, SrlR, StpA, SutR, TorR, UvrY, YdeO, YjjQ, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
SlyA Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Gene name(s): slyA
Functional conformation(s): SlyA
Coregulator(s): ArcA, CRP, DksA-ppGpp, FNR, Fis, Fur, H-NS, IHF, LeuO, LrhA, McbR, PaaX, PhoP, RcdA, SdiA, SlyA, StpA, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
LeuO Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Using internal synthesized signals
Synonym(s): DNA-binding transcriptional dual regulator LeuO
Gene name(s): leuO
Functional conformation(s): LeuO
Coregulator(s): CRP, CadC, DksA-ppGpp, FNR, Fis, H-NS, LeuO, LrhA, MlrA, OmpR, RcsB-BglJ, SlyA, StpA, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
StpA      Tractordb tool
Connectivity class Local Regulator
Synonym(s): DNA-binding transcriptional repressor StpA with RNA chaperone activity, H-NS-like DNA-binding transcriptional repressor with RNA chaperone activity, HnsB
Gene name(s): stpA
Functional conformation(s): StpA
Coregulator(s): CRP, CreB, Fis, H-NS, LeuO, Lrp, MalT, RcsB-BglJ, SlyA, StpA
Summary: TF Summary ...[more]

REGULATION EXERTED BY CRP,H-NS,LeuO,SlyA,StpA Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
CRP repressor H-NS repressor LeuO activator SlyA activator StpA activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
H-NS repressor casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884358 2884372 -46.0 aaagtttcttTAATAATAAAACGAATaacttgcaga   [1]
[2]
[3]
[4]
LeuO activator casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884263 2884283 47.0 tataggttaaTTGTATTAAACCAATGAATATAtttttgcagt   [3]
LeuO activator casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884419 2884439 -110.0 gtatgagattTTATATTCACAGTATGAATATTttatgtaata   [3]
LeuO activator casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884389 2884409 -80.0 ttttatgtaaTAAAATTCATGGTAATTATTATaactaaaagt   [3]
LeuO activator casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884289 2884309 21.0 aacaattcaaCACATCTTAATATATGTATAGGttaattgtat   [3]
CRP-cAMP repressor casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884399 2884419 -89.5 gtatgaatatTTTATGTAATAAAATTCATGGtaattattat   [5]
SlyA activator casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884239 2884238 81.5 tgtgattattGaattaattac   [6]
StpA activator casAp cas2 , cas1 , casE , casD , casC , casB , casA 2884358 2884372 -46.0 aaagtttcttTAATAATAAAACGAATaacttgcaga   [1]
[2]

Reference(s): [1] Pul U., et al., 2010
[2] Sun D., et al., 2020
[3] Westra ER., et al., 2010
[4] Yang D., et al., 2020
[5] Yang CD., et al., 2014
[6] Curran TD., et al., 2017



Reference(s)    

 [1] Pul U., Wurm R., Arslan Z., Geissen R., Hofmann N., Wagner R., 2010, Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS., Mol Microbiol 75(6):1495-512

 [2] Sun D., Mao X., Fei M., Chen Z., Zhu T., Qiu J., 2020, Histone-like Nucleoid-Structuring Protein (H-NS) Paralogue StpA Activates the Type I-E CRISPR-Cas System against Natural Transformation in Escherichia coli., Appl Environ Microbiol 86(14)

 [3] Westra ER., Pul U., Heidrich N., Jore MM., Lundgren M., Stratmann T., Wurm R., Raine A., Mescher M., Van Heereveld L., Mastop M., Wagner EG., Schnetz K., Van Der Oost J., Wagner R., Brouns SJ., 2010, H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO., Mol Microbiol 77(6):1380-93

 [4] Yang D., Wang Z., Ma J., Fu Q., Wu L., Wang H., Wang S., Yan Y., Sun J., 2020, Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/ cas3 Expression to Resist Bacteriophage., Viruses 12(1)

 [5] Yang CD., Chen YH., Huang HY., Huang HD., Tseng CP., 2014, CRP represses the CRISPR/Cas system in Escherichia coli: evidence that endogenous CRISPR spacers impede phage P1 replication., Mol Microbiol 92(5):1072-91

 [6] Curran TD., Abacha F., Hibberd SP., Rolfe MD., Lacey MM., Green J., 2017, Identification of new members of the Escherichia coli K-12 MG1655 SlyA regulon., Microbiology 163(3):400-409


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