RegulonDB RegulonDB 11.0: Regulon Form
   
   


AraC,CRP regulon in Escherichia coli K-12 genome


TRANSCRIPTION FACTOR: Info
Name:
CRP Weight Matrix      Tractordb tool
Connectivity class Global Regulator
Sensing Class Using internal synthesized signals
Synonym(s): Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB
Gene name(s): crp
Functional conformation(s): CRP , CRP-cyclic-AMP
Coregulator(s): AaeR, AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RspR, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp
Summary: TF Summary ...[more]
TRANSCRIPTION FACTOR: Info
Name:
AraC Weight Matrix      Tractordb tool
Connectivity class Local Regulator
Sensing Class Sensing external and internal signals
Synonym(s): DNA-binding transcriptional dual regulator AraC
Gene name(s): araC
Functional conformation(s): AraC-α-L-arabinopyranose , AraC , AraC-D-fucose
Coregulator(s): AraC, CRP, GadX, NagC, XylR
Summary: TF Summary ...[more]

REGULATION EXERTED BY AraC,CRP Info M3D | Colombos
KNOWN BINDING SITES (The central relative position is relative to the promoter +1)    
AraC dual CRP activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
AraC-α-L-arabinopyranose activator araBp araD , araA , araB 70131 70147 -64.0 tgctatgccaTAGCATTTTTATCCATAagattagcgg [GEA] [1]
[2]
[3]
[4]
[5]
AraC repressor araBp araD , araA , araB 70131 70147 -64.0 tgctatgccaTAGCATTTTTATCCATAagattagcgg [GEA] [1]
[2]
[3]
[4]
[5]
CRP-cyclic-AMP activator araBp araD , araA , araB 70158 70179 -93.5 tccacattgaTTATTTGCACGGCGTCACACTTtgctatgcca [GEA] [2]
[6]
[7]
AraC repressor araBp araD , araA , araB 70342 70358 -275.0 attcagagaaGAAACCAATTGTCCATAttgcatcaga   [2]
[3]
[5]
AraC-α-L-arabinopyranose activator araBp araD , araA , araB 70110 70126 -43.0 tccataagatTAGCGGATCCTACCTGAcgctttttat [GEA] [1]
[2]
[3]
[4]
[5]
AraC-α-L-arabinopyranose repressor araBp araD , araA , araB 70205 70221 -138.0 caaaaacgcgTAACAAAAGTGTCTATAatcacggcag [GEA] [8]
AraC-α-L-arabinopyranose repressor araBp araD , araA , araB 70184 70200 -117.0 tctataatcaCGGCAGAAAAGTCCACAttgattattt [GEA] [8]
AraC activator CRP activator    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
CRP-cyclic-AMP activator araFp1 araH , araG , araF 1986270 1986291 -42.5 tgtaattctgCGATGTGATATTGCTCTCCTATggagaattaa [GEA] [9]
[10]
[11]
CRP-cyclic-AMP activator araEp ygeA , araE 2982292 2982313 -94.5 ttaatttgaaAATTGGAATATCCATCACATAAcgacatgtcg   [12]
AraC-α-L-arabinopyranose activator araFp1 araH , araG , araF 1986396 1986412 -166.0 ccaaagacaaCAAGGATTTCCAGGCTAatcttatgga   [10]
[13]
AraC-α-L-arabinopyranose activator araFp1 araH , araG , araF 1986321 1986337 -91.0 ggctttccctTATGTCTTTTCCCGCTAaatttatgca   [10]
[13]
AraC-α-L-arabinopyranose activator araFp1 araH , araG , araF 1986300 1986316 -70.0 ccgctaaattTATGCACGTTCTCACTGtaattctgcg   [10]
[13]
AraC-α-L-arabinopyranose activator araFp1 araH , araG , araF 1986375 1986391 -145.0 aggctaatctTATGGATTAATCTGCTGtgcattcgac   [10]
[13]
AraC-α-L-arabinopyranose activator araEp ygeA , araE 2982265 2982281 -65.0 cgacatgtcgCAGCAATTTAATCCATAtttatgctgt   [12]
AraC-α-L-arabinopyranose activator araEp ygeA , araE 2982244 2982260 -44.0 tccatatttaTGCTGTTTCCGACCTGAcacctgcgtg   [12]
CRP-cyclic-AMP activator araFp3 araH , araG , araF 1986270 1986291 -42.5 tgtaattctgCGATGTGATATTGCTCTCCTATggagaattaa [GEA] [9]
[10]
[11]
AraC-α-L-arabinopyranose activator araFp3 araH , araG , araF 1986396 1986412 -166.0 ccaaagacaaCAAGGATTTCCAGGCTAatcttatgga   [10]
[13]
AraC-α-L-arabinopyranose activator araFp3 araH , araG , araF 1986321 1986337 -91.0 ggctttccctTATGTCTTTTCCCGCTAaatttatgca   [10]
[13]
AraC-α-L-arabinopyranose activator araFp3 araH , araG , araF 1986375 1986391 -145.0 aggctaatctTATGGATTAATCTGCTGtgcattcgac   [10]
[13]
AraC-α-L-arabinopyranose activator araFp3 araH , araG , araF 1986300 1986316 -70.0 ccgctaaattTATGCACGTTCTCACTGtaattctgcg   [10]
[13]
AraC activator CRP dual    
Transcription Factor
Regulated
Binding Sites
Evidence
References
Functional conformation
Function
Promoter
Gene(s)
LeftPos
RigthPos
Central Rel-Pos
Sequence
AraC-α-L-arabinopyranose activator araJp araJ 412585 412601 -64.0 aataactattCAGCAGGATAATGAATAcagaggggcg   [10]
CRP-cyclic-AMP activator araJp araJ 412609 412630 -90.5 cagcaaactgGAAAGTACGTTTGCAGTGAAATaactattcag   [10]
CRP-cyclic-AMP repressor araJp araJ 412475 412497 44.5 gccgttctggTGGTTGTGATGGTGGTATGAAAAaagtcatttt   [10]
AraC-α-L-arabinopyranose activator araJp araJ 412564 412580 -43.0 tgaatacagaGGGGCGAATTATCTCTTggccttgctg   [10]

Evidence: [GEA] Gene expression analysis
Reference(s): [1] Bustos SA., et al., 1993
[2] Lobell RB., et al., 1991
[3] Niland P., et al., 1996
[4] Reeder T., et al., 1993
[5] Seabold RR., et al., 1998
[6] Stoltzfus L., et al., 1989
[7] Zhang X., et al., 1998
[8] Lee DH., et al., 1992
[9] Hendrickson W., et al., 1992
[10] Hendrickson W., et al., 1990
[11] Johnson CM., et al., 2000
[12] Stoner C., et al., 1983
[13] Lu Y., et al., 1992



Reference(s)    

 [1] Bustos SA., Schleif RF., 1993, Functional domains of the AraC protein., Proc Natl Acad Sci U S A 90(12):5638-42

 [2] Lobell RB., Schleif RF., 1991, AraC-DNA looping: orientation and distance-dependent loop breaking by the cyclic AMP receptor protein., J Mol Biol 218(1):45-54

 [3] Niland P., Huhne R., Muller-Hill B., 1996, How AraC interacts specifically with its target DNAs., J Mol Biol 264(4):667-74

 [4] Reeder T., Schleif R., 1993, AraC protein can activate transcription from only one position and when pointed in only one direction., J Mol Biol 231(2):205-18

 [5] Seabold RR., Schleif RF., 1998, Apo-AraC actively seeks to loop., J Mol Biol 278(3):529-38

 [6] Stoltzfus L., Wilcox G., 1989, Effect of mutations in the cyclic AMP receptor protein-binding site on araBAD and araC expression., J Bacteriol 171(2):1178-84

 [7] Zhang X., Schleif R., 1998, Catabolite gene activator protein mutations affecting activity of the araBAD promoter., J Bacteriol 180(2):195-200

 [8] Lee DH., Huo L., Schleif R., 1992, Repression of the araBAD promoter from araO1., J Mol Biol 224(2):335-41

 [9] Hendrickson W., Flaherty C., Molz L., 1992, Sequence elements in the Escherichia coli araFGH promoter., J Bacteriol 174(21):6862-71

 [10] Hendrickson W., Stoner C., Schleif R., 1990, Characterization of the Escherichia coli araFGH and araJ promoters., J Mol Biol 215(4):497-510

 [11] Johnson CM., Schleif RF., 2000, Cooperative action of the catabolite activator protein and AraC in vitro at the araFGH promoter., J Bacteriol 182(7):1995-2000

 [12] Stoner C., Schleif R., 1983, The araE low affinity L-arabinose transport promoter. Cloning, sequence, transcription start site and DNA binding sites of regulatory proteins., J Mol Biol 171(4):369-81

 [13] Lu Y., Flaherty C., Hendrickson W., 1992, AraC protein contacts asymmetric sites in the Escherichia coli araFGH promoter., J Biol Chem 267(34):24848-57


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