RegulonDB

TRANSCRIPTION FACTOR:

Name:

CRP	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB

Gene name(s):

crp

Functional conformation(s):

CRP , CRP-cyclic-AMP

Coregulator(s):

AaeR, AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RspR, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Info

Name:

Lrp	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Sensing external and internal signals

Synonym(s):

AlsB, DNA-binding transcriptional dual regulator Lrp, Ihb, LivR, Lrs, Lss, LstR, Mbf, OppI, RblA

Gene name(s):

lrp

Functional conformation(s):

Lrp , Lrp-L-leucine

Coregulator(s):

AcrR, Ada, AdiY, AidB, ArcA, ArgP, ArgR, CRP, CadC, CpxR, Cra, DksA, DksA-ppGpp, EnvY, FNR, Fis, FliZ, Fur, GadE, GadE-RcsB, GadW, GadX, GcvA, GlaR, H-NS, HU, HdfR, IHF, LrhA, Lrp, MarA, Mlc, ModE, Nac, NarL, NhaR, NsrR, OmpR, PhoP, PurR, QseB, RcsB, Rob, SoxS, StpA, TorR, YeiL, YjjQ, ppGpp

Summary:

TF Summary ...[more]

REGULATION EXERTED BY CRP,Lrp

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
CRP dual Lrp repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
CRP-cyclic-AMP	activator	dadAp1	dadA , dadX	1237518	1237539	20.5	tgagttatcaAGATGTGATTAGATTATTATTCttttactgta	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2]
CRP-cyclic-AMP	activator	dadAp1	dadA , dadX	1237455	1237477	-43.5	agagtcagggAGATGTGAGCCAGCTCACCATAAaaaagccgca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2] [3]
Lrp	repressor	dadAp1	dadA , dadX	1237596	1237610	95.0	ctgggaagtgGTGTGGTAGGCGTTGccagcgcctg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237574	1237588	73.0	agtggctatgCGAGTTGTCATACTGggaagtggtg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237527	1237541	26.0	aagatgtgatTAGATTATTATTCTTttactgtatc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237495	1237509	-7.0	gcatgttgaaTAATATTTTCAACTGagttatcaag	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237450	1237464	-52.0	gccgcagagtCAGGGAGATGTGAGCcagctcacca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237412	1237426	-90.0	aaagggcgttCAGCAGGAGATACTAaagacgccat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237382	1237396	-120.0	cgcttctgaaCGGAATTTTATGCTGgataaaaagg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237356	1237370	-146.0	ttcttattatCAGAGTGCCACTAATccgcttctga	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237325	1237339	-177.0	cgcgtcgccgGAGAATTTTTTTCTTtgcgatttct	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237277	1237291	-225.0	tctctgtgtgGAGCACCTCATTTCAagcatagaac	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp1	dadA , dadX	1237252	1237266	-250.0	acgcgcacctCATTGTTGTCGGCGCtctctgtgtg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
CRP-cyclic-AMP	repressor	dadAp1	dadA , dadX	1237518	1237539	20.5	tgagttatcaAGATGTGATTAGATTATTATTCttttactgta	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2]
CRP repressor Lrp activator
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
Lrp	activator	serCp1	serC , aroA	957454	957468	-134.0	gggtgacctgACAGTAAAAACATCGgctttttgct	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [6]
CRP-cyclic-AMP	repressor	serCp1	serC , aroA	957498	957519	-86.0	agagattcttTTGTGTGATGCAAGCCACATTTttgccctcaa	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[7] [6]
CRP activator Lrp repressor
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
CRP-cyclic-AMP	activator	dadAp3	dadA , dadX	1237518	1237539	-9.5	tgagttatcaAGATGTGATTAGATTATTATTCttttactgta	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2] [3]
CRP-cyclic-AMP	activator	dadAp3	dadA , dadX	1237455	1237477	-72.5	agagtcagggAGATGTGAGCCAGCTCACCATAAaaaagccgca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2] [3]
Lrp	repressor	dadAp3	dadA , dadX	1237596	1237610	66.0	ctgggaagtgGTGTGGTAGGCGTTGccagcgcctg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237574	1237588	44.0	agtggctatgCGAGTTGTCATACTGggaagtggtg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237527	1237541	-4.0	aagatgtgatTAGATTATTATTCTTttactgtatc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237495	1237509	-36.0	gcatgttgaaTAATATTTTCAACTGagttatcaag	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237450	1237464	-81.0	gccgcagagtCAGGGAGATGTGAGCcagctcacca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
CRP-cyclic-AMP	activator	dadAp2	dadA , dadX	1237520	1237541	7.0	agttatcaagATGTGATTAGATTATTATTCTTttactgtatc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]
CRP-cyclic-AMP	activator	dadAp2	dadA , dadX	1237455	1237477	-59.5	agagtcagggAGATGTGAGCCAGCTCACCATAAaaaagccgca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2] [3]
Lrp	repressor	dadAp2	dadA , dadX	1237596	1237610	79.0	ctgggaagtgGTGTGGTAGGCGTTGccagcgcctg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237574	1237588	57.0	agtggctatgCGAGTTGTCATACTGggaagtggtg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237527	1237541	10.0	aagatgtgatTAGATTATTATTCTTttactgtatc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237495	1237509	-23.0	gcatgttgaaTAATATTTTCAACTGagttatcaag	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237450	1237464	-68.0	gccgcagagtCAGGGAGATGTGAGCcagctcacca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
CRP-cyclic-AMP	activator	serAp2	serA	3058618	3058639	-80.0	cccccgttaaAAAAATTCTCTTCATTAAATTTggtgacatgt	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[8]
Lrp	repressor	dadAp3	dadA , dadX	1237412	1237426	-119.0	aaagggcgttCAGCAGGAGATACTAaagacgccat	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ] [EXP-IMP-SITE-MUTATION]	[4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237382	1237396	-149.0	cgcttctgaaCGGAATTTTATGCTGgataaaaagg	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ] [EXP-IMP-SITE-MUTATION]	[5] [4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237356	1237370	-175.0	ttcttattatCAGAGTGCCACTAATccgcttctga	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ] [EXP-IMP-SITE-MUTATION]	[4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237325	1237339	-206.0	cgcgtcgccgGAGAATTTTTTTCTTtgcgatttct	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ] [EXP-IMP-SITE-MUTATION]	[5] [4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237277	1237291	-254.0	tctctgtgtgGAGCACCTCATTTCAagcatagaac	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ] [EXP-IMP-SITE-MUTATION]	[4] [1] [2]
Lrp	repressor	dadAp3	dadA , dadX	1237252	1237266	-279.0	acgcgcacctCATTGTTGTCGGCGCtctctgtgtg	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ] [EXP-IMP-SITE-MUTATION]	[4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237412	1237426	-106.0	aaagggcgttCAGCAGGAGATACTAaagacgccat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237382	1237396	-136.0	cgcttctgaaCGGAATTTTATGCTGgataaaaagg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237356	1237370	-162.0	ttcttattatCAGAGTGCCACTAATccgcttctga	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237325	1237339	-193.0	cgcgtcgccgGAGAATTTTTTTCTTtgcgatttct	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[5] [4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237277	1237291	-241.0	tctctgtgtgGAGCACCTCATTTCAagcatagaac	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp	repressor	dadAp2	dadA , dadX	1237252	1237266	-266.0	acgcgcacctCATTGTTGTCGGCGCtctctgtgtg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[4] [1] [2]
Lrp-L-leucine	repressor	serAp2	serA

Evidence:

[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
[EXP-IEP-RNA-SEQ] high throughput RNA-seq evidence
[EXP-IEP-MICROARRAY] high throughput expression microarray evidence
[COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins
[EXP-IMP-SITE-MUTATION] Site mutation

Reference(s):

[1] Mathew E., et al., 1996
[2] Zhi J., et al., 1999
[3] Zhi J., et al., 1998
[4] Kroner GM., et al., 2019
[5] Cho BK., et al., 2008
[6] Man TK., et al., 1997
[7] Lim CJ., et al., 1994
[8] Yang L., et al., 2002

Reference(s)
[1] Mathew E., Zhi J., Freundlich M., 1996, Lrp is a direct repressor of the dad operon in Escherichia coli., J Bacteriol 178(24):7234-40
[2] Zhi J., Mathew E., Freundlich M., 1999, Lrp binds to two regions in the dadAX promoter region of Escherichia coli to repress and activate transcription directly., Mol Microbiol 32(1):29-40
[3] Zhi J., Mathew E., Freundlich M., 1998, In vitro and in vivo characterization of three major dadAX promoters in Escherichia coli that are regulated by cyclic AMP-CRP and Lrp., Mol Gen Genet 258(4):442-7
[4] Kroner GM., Wolfe MB., Freddolino PL., 2019, Escherichia coli Lrp Regulates One-Third of the Genome via Direct, Cooperative, and Indirect Routes., J Bacteriol 201(3)
[5] Cho BK., Barrett CL., Knight EM., Park YS., Palsson BO., 2008, Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli., Proc Natl Acad Sci U S A 105(49):19462-7
[6] Man TK., Pease AJ., Winkler ME., 1997, Maximization of transcription of the serC (pdxF)-aroA multifunctional operon by antagonistic effects of the cyclic AMP (cAMP) receptor protein-cAMP complex and Lrp global regulators of Escherichia coli K-12., J Bacteriol 179(11):3458-69
[7] Lim CJ., Hwang W., Park EH., Fuchs JA., 1994, Cyclic AMP-dependent expression of the Escherichia coli serC-aroA operon., Biochim Biophys Acta 1218(2):250-3
[8] Yang L., Lin RT., Newman EB., 2002, Structure of the Lrp-regulated serA promoter of Escherichia coli K-12., Mol Microbiol 43(2):323-33