RegulonDB

TRANSCRIPTION FACTOR:

Name:

CRP	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB

Gene name(s):

crp

Functional conformation(s):

CRP , CRP-cyclic-AMP

Coregulator(s):

AaeR, AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RspR, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Info

Name:

AraC	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

Sensing external and internal signals

Synonym(s):

DNA-binding transcriptional dual regulator AraC

Gene name(s):

araC

Functional conformation(s):

AraC-α-L-arabinopyranose , AraC , AraC-D-fucose

Coregulator(s):

AraC, CRP, GadX, NagC, XylR

Summary:

TF Summary ...[more]

REGULATION EXERTED BY AraC,CRP

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
AraC dual CRP activator
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
AraC-α-L-arabinopyranose	activator	araBp	araD , araA , araB	70131	70147	-64.0	tgctatgccaTAGCATTTTTATCCATAagattagcgg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2] [3] [4] [5]
AraC	repressor	araBp	araD , araA , araB	70131	70147	-64.0	tgctatgccaTAGCATTTTTATCCATAagattagcgg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2] [3] [4] [5]
CRP-cyclic-AMP	activator	araBp	araD , araA , araB	70158	70179	-93.5	tccacattgaTTATTTGCACGGCGTCACACTTtgctatgcca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [6] [7]
AraC	repressor	araBp	araD , araA , araB	70342	70358	-275.0	attcagagaaGAAACCAATTGTCCATAttgcatcaga		[2] [3] [5]
AraC-α-L-arabinopyranose	activator	araBp	araD , araA , araB	70110	70126	-43.0	tccataagatTAGCGGATCCTACCTGAcgctttttat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1] [2] [3] [4] [5]
AraC-α-L-arabinopyranose	repressor	araBp	araD , araA , araB	70205	70221	-138.0	caaaaacgcgTAACAAAAGTGTCTATAatcacggcag	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[8]
AraC-α-L-arabinopyranose	repressor	araBp	araD , araA , araB	70184	70200	-117.0	tctataatcaCGGCAGAAAAGTCCACAttgattattt	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[8]
AraC activator CRP activator
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
CRP-cyclic-AMP	activator	araFp1	araH , araG , araF	1986270	1986291	-42.5	tgtaattctgCGATGTGATATTGCTCTCCTATggagaattaa	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[9] [10] [11]
CRP-cyclic-AMP	activator	araEp	ygeA , araE	2982292	2982313	-94.5	ttaatttgaaAATTGGAATATCCATCACATAAcgacatgtcg		[12]
AraC-α-L-arabinopyranose	activator	araFp1	araH , araG , araF	1986396	1986412	-166.0	ccaaagacaaCAAGGATTTCCAGGCTAatcttatgga		[10] [13]
AraC-α-L-arabinopyranose	activator	araFp1	araH , araG , araF	1986321	1986337	-91.0	ggctttccctTATGTCTTTTCCCGCTAaatttatgca		[10] [13]
AraC-α-L-arabinopyranose	activator	araFp1	araH , araG , araF	1986300	1986316	-70.0	ccgctaaattTATGCACGTTCTCACTGtaattctgcg		[10] [13]
AraC-α-L-arabinopyranose	activator	araFp1	araH , araG , araF	1986375	1986391	-145.0	aggctaatctTATGGATTAATCTGCTGtgcattcgac		[10] [13]
AraC-α-L-arabinopyranose	activator	araEp	ygeA , araE	2982265	2982281	-65.0	cgacatgtcgCAGCAATTTAATCCATAtttatgctgt		[12]
AraC-α-L-arabinopyranose	activator	araEp	ygeA , araE	2982244	2982260	-44.0	tccatatttaTGCTGTTTCCGACCTGAcacctgcgtg		[12]
CRP-cyclic-AMP	activator	araFp3	araH , araG , araF	1986270	1986291	-42.5	tgtaattctgCGATGTGATATTGCTCTCCTATggagaattaa	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[9] [10] [11]
AraC-α-L-arabinopyranose	activator	araFp3	araH , araG , araF	1986396	1986412	-166.0	ccaaagacaaCAAGGATTTCCAGGCTAatcttatgga		[10] [13]
AraC-α-L-arabinopyranose	activator	araFp3	araH , araG , araF	1986321	1986337	-91.0	ggctttccctTATGTCTTTTCCCGCTAaatttatgca		[10] [13]
AraC-α-L-arabinopyranose	activator	araFp3	araH , araG , araF	1986375	1986391	-145.0	aggctaatctTATGGATTAATCTGCTGtgcattcgac		[10] [13]
AraC-α-L-arabinopyranose	activator	araFp3	araH , araG , araF	1986300	1986316	-70.0	ccgctaaattTATGCACGTTCTCACTGtaattctgcg		[10] [13]
AraC activator CRP dual
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
AraC-α-L-arabinopyranose	activator	araJp	araJ	412585	412601	-64.0	aataactattCAGCAGGATAATGAATAcagaggggcg		[10]
CRP-cyclic-AMP	activator	araJp	araJ	412609	412630	-90.5	cagcaaactgGAAAGTACGTTTGCAGTGAAATaactattcag		[10]
CRP-cyclic-AMP	repressor	araJp	araJ	412475	412497	44.5	gccgttctggTGGTTGTGATGGTGGTATGAAAAaagtcatttt		[10]
AraC-α-L-arabinopyranose	activator	araJp	araJ	412564	412580	-43.0	tgaatacagaGGGGCGAATTATCTCTTggccttgctg		[10]

Evidence:	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
Reference(s):	[1] Bustos SA., et al., 1993 [2] Lobell RB., et al., 1991 [3] Niland P., et al., 1996 [4] Reeder T., et al., 1993 [5] Seabold RR., et al., 1998 [6] Stoltzfus L., et al., 1989 [7] Zhang X., et al., 1998 [8] Lee DH., et al., 1992 [9] Hendrickson W., et al., 1992 [10] Hendrickson W., et al., 1990 [11] Johnson CM., et al., 2000 [12] Stoner C., et al., 1983 [13] Lu Y., et al., 1992

Reference(s)
[1] Bustos SA., Schleif RF., 1993, Functional domains of the AraC protein., Proc Natl Acad Sci U S A 90(12):5638-42
[2] Lobell RB., Schleif RF., 1991, AraC-DNA looping: orientation and distance-dependent loop breaking by the cyclic AMP receptor protein., J Mol Biol 218(1):45-54
[3] Niland P., Huhne R., Muller-Hill B., 1996, How AraC interacts specifically with its target DNAs., J Mol Biol 264(4):667-74
[4] Reeder T., Schleif R., 1993, AraC protein can activate transcription from only one position and when pointed in only one direction., J Mol Biol 231(2):205-18
[5] Seabold RR., Schleif RF., 1998, Apo-AraC actively seeks to loop., J Mol Biol 278(3):529-38
[6] Stoltzfus L., Wilcox G., 1989, Effect of mutations in the cyclic AMP receptor protein-binding site on araBAD and araC expression., J Bacteriol 171(2):1178-84
[7] Zhang X., Schleif R., 1998, Catabolite gene activator protein mutations affecting activity of the araBAD promoter., J Bacteriol 180(2):195-200
[8] Lee DH., Huo L., Schleif R., 1992, Repression of the araBAD promoter from araO1., J Mol Biol 224(2):335-41
[9] Hendrickson W., Flaherty C., Molz L., 1992, Sequence elements in the Escherichia coli araFGH promoter., J Bacteriol 174(21):6862-71
[10] Hendrickson W., Stoner C., Schleif R., 1990, Characterization of the Escherichia coli araFGH and araJ promoters., J Mol Biol 215(4):497-510
[11] Johnson CM., Schleif RF., 2000, Cooperative action of the catabolite activator protein and AraC in vitro at the araFGH promoter., J Bacteriol 182(7):1995-2000
[12] Stoner C., Schleif R., 1983, The araE low affinity L-arabinose transport promoter. Cloning, sequence, transcription start site and DNA binding sites of regulatory proteins., J Mol Biol 171(4):369-81
[13] Lu Y., Flaherty C., Hendrickson W., 1992, AraC protein contacts asymmetric sites in the Escherichia coli araFGH promoter., J Biol Chem 267(34):24848-57