RegulonDB

TRANSCRIPTION FACTOR:

Name:

H-NS	Tractordb tool

Connectivity class

Global Regulator

Sensing Class

TFs for DNA-bending

Synonym(s):

BglY, Cur, DNA-binding protein HLP-II (HU, BH2, HD, NS), DNA-binding transcriptional dual regulator H-NS, Drc, DrdX, Drs, FimG, Hns, HnsA, Irk, MsyA, OsmZ, PilG, TopS, TopX, VirR

Gene name(s):

hns

Functional conformation(s):

H-NS

Coregulator(s):

AcrR, AdiY, ArcA, ArgP, ArgR, BasR, BolA, BtsR, CRP, CadC, CaiF, CdaR, CpxR, Cra, CreB, CsgD, CspA, CysB, DksA, DksA-ppGpp, DnaA, DpiA, EvgA, FNR, Fis, FlhDC, FliZ, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GlaR, GutM, H-NS, HU, HdfR, HypT, IHF, LacI, LeuO, LrhA, Lrp, MalT, MarA, MatA, McbR, MlrA, MntR, MqsA, NagC, NanR, NarL, NarP, NhaR, NrdR, NsrR, OmpR, OxyR, PhoB, PhoP, QseB, RcdA, RcsAB, RcsB, RcsB-BglJ, Rob, RstA, RutR, SdiA, SlyA, SoxS, SrlR, StpA, SutR, TorR, UvrY, YdeO, YjjQ, ppGpp

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Info

Name:

Lrp	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Sensing external and internal signals

Synonym(s):

AlsB, DNA-binding transcriptional dual regulator Lrp, Ihb, LivR, Lrs, Lss, LstR, Mbf, OppI, RblA

Gene name(s):

lrp

Functional conformation(s):

Lrp , Lrp-L-leucine

Coregulator(s):

AcrR, Ada, AdiY, AidB, ArcA, ArgP, ArgR, CRP, CadC, CpxR, Cra, DksA, DksA-ppGpp, EnvY, FNR, Fis, FliZ, Fur, GadE, GadE-RcsB, GadW, GadX, GcvA, GlaR, H-NS, HU, HdfR, IHF, LrhA, Lrp, MarA, Mlc, ModE, Nac, NarL, NhaR, NsrR, OmpR, PhoP, PurR, QseB, RcsB, Rob, SoxS, StpA, TorR, YeiL, YjjQ, ppGpp

Summary:

TF Summary ...[more]

REGULATION EXERTED BY H-NS,Lrp

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
H-NS repressor Lrp activator
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
Lrp	activator	ilvIHp1	ilvI , ilvH	85341	85355	-252.0	agacatgaatGTCTGGTTTATTCTGcattttttat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Lrp	activator	ilvIHp1	ilvI , ilvH	85372	85386	-221.0	ttattgaatgTAGAATTTTATTCTGaatgtgtggg	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IEP-RNA-SEQ]	[12] [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Lrp	activator	ilvIHp1	ilvI , ilvH	85453	85467	-140.0	ttgtgggattCAGCCGATTTATTATcaatttaatc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Lrp	activator	ilvIHp1	ilvI , ilvH	85488	85502	-105.0	ctctgtaatgGAGGATTTTATCGTTtcttttcacc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Lrp	activator	ilvIHp1	ilvI , ilvH	85517	85531	-76.0	ttcacctttcCTCCTGTTTATTCTTattaccccgt	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Lrp	activator	ilvIHp1	ilvI , ilvH	85537	85551	-56.0	ttcttattacCCCGTGTTTATGTCTctggctgcca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-RNA-SEQ]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11]
Lrp	activator	osmCp2	osmC	1556537	1556551	-62.0	aatattttcgCCGGATTTTATTCGGaatatcctgc	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[13]
H-NS	repressor	osmCp2	osmC

Evidence:	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis [EXP-IEP-RNA-SEQ] high throughput RNA-seq evidence [EXP-IEP-MICROARRAY] high throughput expression microarray evidence
Reference(s):	[1] Haughn GW., et al., 1985 [2] Jafri S., et al., 2002 [3] Kroner GM., et al., 2019 [4] Marasco R., et al., 1994 [5] Platko JV., et al., 1993 [6] Platko JV., et al., 1990 [7] Ricca E., et al., 1989 [8] Wang Q., et al., 1993 [9] Wang Q., et al., 1993 [10] Wang Q., et al., 1993 [11] Willins DA., et al., 1992 [12] Cho BK., et al., 2008 [13] Bouvier J., et al., 1998

Reference(s)
[1] Haughn GW., Squires CH., De Felice M., Largo CT., Calvo JM., 1985, Unusual organization of the ilvIH promoter of Escherichia coli., J Bacteriol 163(1):186-98
[2] Jafri S., Chen S., Calvo JM., 2002, ilvIH operon expression in Escherichia coli requires Lrp binding to two distinct regions of DNA., J Bacteriol 184(19):5293-300
[3] Kroner GM., Wolfe MB., Freddolino PL., 2019, Escherichia coli Lrp Regulates One-Third of the Genome via Direct, Cooperative, and Indirect Routes., J Bacteriol 201(3)
[4] Marasco R., Varcamonti M., La Cara F., Ricca E., De Felice M., Sacco M., 1994, In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli., J Bacteriol 176(17):5197-201
[5] Platko JV., Calvo JM., 1993, Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine., J Bacteriol 175(4):1110-7
[6] Platko JV., Willins DA., Calvo JM., 1990, The ilvIH operon of Escherichia coli is positively regulated., J Bacteriol 172(8):4563-70
[7] Ricca E., Aker DA., Calvo JM., 1989, A protein that binds to the regulatory region of the Escherichia coli ilvIH operon., J Bacteriol 171(3):1658-64
[8] Wang Q., Calvo JM., 1993, Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH., J Mol Biol 229(2):306-18
[9] Wang Q., Calvo JM., 1993, Lrp, a major regulatory protein in Escherichia coli, bends DNA and can organize the assembly of a higher-order nucleoprotein structure., EMBO J 12(6):2495-501
[10] Wang Q., Sacco M., Ricca E., Lago CT., De Felice M., Calvo JM., 1993, Organization of Lrp-binding sites upstream of ilvIH in Salmonella typhimurium., Mol Microbiol 7(6):883-91
[11] Willins DA., Calvo JM., 1992, In vitro transcription from the Escherichia coli ilvIH promoter., J Bacteriol 174(23):7648-55
[12] Cho BK., Barrett CL., Knight EM., Park YS., Palsson BO., 2008, Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli., Proc Natl Acad Sci U S A 105(49):19462-7
[13] Bouvier J., Gordia S., Kampmann G., Lange R., Hengge-Aronis R., Gutierrez C., 1998, Interplay between global regulators of Escherichia coli: effect of RpoS, Lrp and H-NS on transcription of the gene osmC., Mol Microbiol 28(5):971-80
[14] Levinthal M., Lejeune P., Danchin A., 1994, The H-NS protein modulates the activation of the ilvIH operon of Escherichia coli K12 by Lrp, the leucine regulatory protein., Mol Gen Genet 242(6):736-43