RegulonDB

TRANSCRIPTION FACTOR:

Name:

Lrp	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Sensing external and internal signals

Synonym(s):

AlsB, DNA-binding transcriptional dual regulator Lrp, Ihb, LivR, Lrs, Lss, LstR, Mbf, OppI, RblA

Gene name(s):

lrp

Functional conformation(s):

Lrp , Lrp-L-leucine

Coregulator(s):

AcrR, Ada, AdiY, AidB, ArcA, ArgP, ArgR, CRP, CadC, CpxR, Cra, DksA, DksA-ppGpp, EnvY, FNR, Fis, FliZ, Fur, GadE, GadE-RcsB, GadW, GadX, GcvA, GlaR, H-NS, HU, HdfR, IHF, LrhA, Lrp, MarA, Mlc, ModE, Nac, NarL, NhaR, NsrR, OmpR, PhoP, PurR, QseB, RcsB, Rob, SoxS, StpA, TorR, YeiL, YjjQ, ppGpp

Summary:

TF Summary ...[more]

REGULATION EXERTED BY Lrp,ppGpp

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
Lrp repressor ppGpp inhibition
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
Lrp	repressor	ilvIp2	ilvI , ilvH	85517	85531	-16.0	ttcacctttcCTCCTGTTTATTCTTattaccccgt	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IMP-SITE-MUTATION]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
Lrp	repressor	ilvIp2	ilvI , ilvH	85537	85551	5.0	ttcttattacCCCGTGTTTATGTCTctggctgcca	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IMP-SITE-MUTATION]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
Lrp	repressor	ilvIp2	ilvI , ilvH	85488	85502	-45.0	ctctgtaatgGAGGATTTTATCGTTtcttttcacc	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IMP-SITE-MUTATION]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
Lrp	repressor	ilvIp2	ilvI , ilvH	85341	85355	-192.0	agacatgaatGTCTGGTTTATTCTGcattttttat	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IMP-SITE-MUTATION]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
Lrp	repressor	ilvIp2	ilvI , ilvH	85372	85386	-161.0	ttattgaatgTAGAATTTTATTCTGaatgtgtggg	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IEP-MICROARRAY] [EXP-IMP-SITE-MUTATION]	[11] [1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
Lrp	repressor	ilvIp2	ilvI , ilvH	85453	85467	-80.0	ttgtgggattCAGCCGATTTATTATcaatttaatc	[COMP-HINF-SIMILAR-TO-CONSENSUS] [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] [EXP-IEP-GENE-EXPRESSION-ANALYSIS] [EXP-IMP-SITE-MUTATION]	[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]

Evidence:

[COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.
[EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts
[EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins
[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
[EXP-IMP-SITE-MUTATION] Site mutation
[EXP-IEP-MICROARRAY] high throughput expression microarray evidence

Reference(s):

[1] Haughn GW., et al., 1985
[2] Jafri S., et al., 2002
[3] Marasco R., et al., 1994
[4] Platko JV., et al., 1993
[5] Platko JV., et al., 1990
[6] Ricca E., et al., 1989
[7] Wang Q., et al., 1993
[8] Wang Q., et al., 1993
[9] Wang Q., et al., 1993
[10] Willins DA., et al., 1992
[11] Cho BK., et al., 2008

Reference(s)
[1] Haughn GW., Squires CH., De Felice M., Largo CT., Calvo JM., 1985, Unusual organization of the ilvIH promoter of Escherichia coli., J Bacteriol 163(1):186-98
[2] Jafri S., Chen S., Calvo JM., 2002, ilvIH operon expression in Escherichia coli requires Lrp binding to two distinct regions of DNA., J Bacteriol 184(19):5293-300
[3] Marasco R., Varcamonti M., La Cara F., Ricca E., De Felice M., Sacco M., 1994, In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli., J Bacteriol 176(17):5197-201
[4] Platko JV., Calvo JM., 1993, Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine., J Bacteriol 175(4):1110-7
[5] Platko JV., Willins DA., Calvo JM., 1990, The ilvIH operon of Escherichia coli is positively regulated., J Bacteriol 172(8):4563-70
[6] Ricca E., Aker DA., Calvo JM., 1989, A protein that binds to the regulatory region of the Escherichia coli ilvIH operon., J Bacteriol 171(3):1658-64
[7] Wang Q., Calvo JM., 1993, Lrp, a global regulatory protein of Escherichia coli, binds co-operatively to multiple sites and activates transcription of ilvIH., J Mol Biol 229(2):306-18
[8] Wang Q., Calvo JM., 1993, Lrp, a major regulatory protein in Escherichia coli, bends DNA and can organize the assembly of a higher-order nucleoprotein structure., EMBO J 12(6):2495-501
[9] Wang Q., Sacco M., Ricca E., Lago CT., De Felice M., Calvo JM., 1993, Organization of Lrp-binding sites upstream of ilvIH in Salmonella typhimurium., Mol Microbiol 7(6):883-91
[10] Willins DA., Calvo JM., 1992, In vitro transcription from the Escherichia coli ilvIH promoter., J Bacteriol 174(23):7648-55
[11] Cho BK., Barrett CL., Knight EM., Park YS., Palsson BO., 2008, Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli., Proc Natl Acad Sci U S A 105(49):19462-7