RegulonDB RegulonDB 10.8: smallRNA Form
   

MgrR small RNA in Escherichia coli K-12 genome


Gene local context to scale (view description)

dgcZ mgtT mgrR mgtS PhoP mgrRp mgrRp
small RNA      
Gene name: mgrR    Texpresso search in the literature
Synonym(s): MgrR small regulatory RNA, small regulatory RNA MgrR
Genome position: 1622817 <-- 1622914
Strand: reverse
Sequence: Get ribonucleotide sequence FastaFormat
GC content %:  
41.84
Note(s): MgrR is a conserved small regulatory RNA that may affect lipopolysaccharide composition Moon K,2009. Potential regulatory targets of MgrR were identified by RIL-seq Melamed S,2016. MgrR negatively regulates the levels of SoxS 27137887 and the cation-phosphate symporter PitA 30276893.
MgrR was first identified in an intergenic region with a high Hfq co-immunoprecipitation signal on microarrays Zhang A,2003. MgrR is highly expressed in rich growth media and may be stabilized by Hfq; expression is positively regulated by the PhoPQ system in response to Mg2+ concentration Moon K,2009. At high Ca2+ levels, expression of MgrR is significantly decreased Moon K,2013.
MgrR was classified as a Class II small RNA, which accumulate to higher levels in the rim mutants and to reduced levels in distal face mutants of Hfq 26373314. Small RNAs compete for binding to Hfq. Expression of OxyS at physiologically relevant levels reduces the abundance of other small RNAs including MgrR and reduces their association with Hfq. Increased levels of Hfq overcome this competition effect Moon K,2011. The interaction of MgrR with Hfq and its effect on the interaction of MgrR with target mRNAs has been studied in more detail 30217864.
An mgrR mutant is ten times more resistant to Polymyxin B than wild type, likely due to the effect of MgrR on eptB expression Moon K,2009.
MgrR: Mg2+ responsive RNA Moon K,2009
Reviews: 19906183. 26618164. Frohlich KS,2018
Evidence: [IDA] Inferred from direct assay
[IHBCE] Inferred by a human based on computational evidence
[IMP] Inferred from mutant phenotype
Reference(s): [1] Moon K., et al., 2009
External database links:  
ECOCYC:
RNA0-348
M3D: MgrR


Regulation exerted by the small RNA    
  Target Regulation Type Mechanism Function Binding Site Evidence
Code
Reference(s)
LeftPos RightPos Distance
to the gene
Sequence
 
base-pairing
post-transcriptional regulation
repressor
3710468
3710482
0
TGTTTGCATGAGAT
 
 
base-pairing
post-transcriptional regulation
repressor
2970426
2970437
11
TTGCATGGATA
 
Evidence: [GEA] Gene expression analysis
[HIFS] Human inference of function from sequence
[IMP] Inferred from mutant phenotype
[IPI] Inferred from physical interaction


Reference(s)    

 [1] Moon K., Gottesman S., 2009, A PhoQ/P-regulated small RNA regulates sensitivity of Escherichia coli to antimicrobial peptides., Mol Microbiol 74(6):1314-30


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